Kinetic spectra of light-adaptation dark-adaptation and M-intermediate of BR-D96N

doi:10.1016/S0030-4018(03)01161-1

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	Kinetic spectra of light-adaptation dark-adaptation and M-intermediate of BR-D96N
	Yao, BL ; Zheng, YA ; Wang, YL ; Lei, M ; Chen, GF ; Hampp, N ; Yao Baoli
作者部门	瞬态光学国家重点实验室
	2003-03-15
发表期刊	OPTICS COMMUNICATIONS
ISSN	0030-4018
卷号	218 期号:1-3 页码:125-130
摘要	BR-D96N is a kind of genetically site-specific mutants of bacteriorhodopsin (BR) with obvious photochromic effect. Compared to the wild type BR, the lifetime of M state of BR-D96N is prolonged to several minutes so that the photochromic kinetics and the intermediates formation can be studied by the conventional spectra analysis. In the experiment, the absorption spectra of the sample at different time after light illumination are measured with spectrophotometer. By fitting and analyzing the variation of the spectra, we suppose that there are three main states in the, photochromic process, i.e., B state (light-adapted state), M state and D state (dark-adapted state). The absorption spectra of the B state, M state and D state are extracted from the experimental data based on this three-state model and the spectra at various time are fitted with the least-square method. So, the variations of population percentages of the M state, B state and D state are obtained and the M state and B state lifetimes are estimated. In another way, from the measurement of the absorption dynamics at 407 and 568 nm, the M state and B state lifetimes are also obtained by two exponential data fitting, which give coincident results with those of the spectra analysis. (C) 2003 Elsevier Science B.V. All rights reserved.
文章类型	Article
关键词	Bacteriorhodopsin Br-d96n Photochromic Kinetic Spectra
学科领域	光学
WOS标题词	Science & Technology ; Physical Sciences
DOI	10.1016/S0030-4018(03)01161-1
收录类别	SCI ; EI
关键词[WOS]	BACTERIORHODOPSIN
语种	英语
WOS研究方向	Optics
WOS类目	Optics
WOS记录号	WOS:000181641000017
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.opt.ac.cn/handle/181661/6478
专题	瞬态光学研究室
通讯作者	Yao Baoli
作者单位	1.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt Technol, Xian 710068, Peoples R China 2.Univ Marburg, Inst Chem Phys, D-35032 Marburg, Germany
推荐引用方式 GB/T 7714	Yao, BL,Zheng, YA,Wang, YL,et al. Kinetic spectra of light-adaptation dark-adaptation and M-intermediate of BR-D96N[J]. OPTICS COMMUNICATIONS,2003,218(1-3):125-130.
APA	Yao, BL.,Zheng, YA.,Wang, YL.,Lei, M.,Chen, GF.,...&Yao Baoli.(2003).Kinetic spectra of light-adaptation dark-adaptation and M-intermediate of BR-D96N.OPTICS COMMUNICATIONS,218(1-3),125-130.
MLA	Yao, BL,et al."Kinetic spectra of light-adaptation dark-adaptation and M-intermediate of BR-D96N".OPTICS COMMUNICATIONS 218.1-3(2003):125-130.

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