Solid-phase binding assay system and method for interferometrically measuring analytes bound to an active receptor

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	Solid-phase binding assay system and method for interferometrically measuring analytes bound to an active receptor
其他题名	Solid-phase binding assay system and method for interferometrically measuring analytes bound to an active receptor
	ALLEN, JIMMY D.; COBB, MICHAEL E.; GUSTAFSON, ERIC K
	1995-01-24
专利权人	FIRST MEDICAL, INC.
公开日期	1995-01-24
授权国家	澳大利亚
专利类型	发明申请
摘要	Apparatus (10) and method for detecting and measuring analyte in biological and other sample solutions. A substrate (20) is provided with at least one active region which specifically binds analyte and an inactive region that does not, and a change in optical path length through the active region due to binding of analyte is measured. The substrate (20) is inserted into one or both beams of an interferometer (10) and moved relative to the interferometer (10). As the substrate (20) moves, the active and inactive regions pass successively through the beam(s) causing a periodic phase shift in the light. When the beams, one or both of which have undergone phase modulation, are recombined with each other, the phase modulation is converted into an amplitude modulation. Each recombined beam is directed to a photodetector (42, 45), which converts the periodically varying optical power into a periodically varying electrical signal. This signal has an amplitude proportional to the amount of bound analyte on the surface of the substrate (20) and a signal frequency equal to that at which the active regions move past the beam(s) in the interferometer (10). Servo control (60, 62, 65) is used to maintain the interferometer at a desired operating point that maximizes the sensitivity and linearity of the system so that the small phase differences due to analyte binding can be measured more accurately.
其他摘要	装置（10）和用于检测和测量生物和其他样品溶液中的分析物的方法。基板（20）设置有至少一个特异性结合分析物的活性区域和不具有非活性区域的活性区域，并且测量由于分析物的结合而通过活性区域的光学路径长度的变化。将基板（20）插入干涉仪（10）的一个或两个光束中并相对于干涉仪（10）移动。随着衬底（20）移动，有源区和无源区连续通过光束，导致光中的周期性相移。当其中一个或两个已经经过相位调制的光束彼此重新组合时，相位调制被转换成幅度调制。每个重新组合的光束被引导到光电探测器（42,45），光电探测器将周期性变化的光功率转换成周期性变化的电信号。该信号的幅度与基板（20）表面上的结合分析物的量成比例，并且信号频率等于有源区域在干涉仪（10）中移过光束的信号频率。伺服控制（60,62,65）用于将干涉仪维持在所需的操作点，以最大化系统的灵敏度和线性度，从而可以更准确地测量由于分析物结合导致的小的相位差。
主权项	For use with a substrate having (a) at least one active region having thereon an immobilized substance that specifically binds an analyte and (b) at least one inactive region, an interferometer-based system for measuring analyte bound to said active region, comprising: a light source for outputting a source beam; first means for splitting said source beam into first and second beams directed along first and second light paths, respectively, with at least one of said first and second beams encountering said substrate; means for effecting relative movement between said substrate and said first and second light paths so that at least one of said first and second beams encounters said active and inactive regions and passes through analyte bound to said active region, wherein at least one of said first and second beams undergoes a phase change that depends on the amount of analyte bound to said active region as a result of encountering said active and inactive regions; second means for recombining said first and second beams after said at least one of said first and second beams encounters said active region, said first beam accumulating a first total phase along said first path between said first means and said second means, said second beam accumulating a second total phase along said second path between said first means and said second means; said second means providing at least one recombined beam having optical power that depends on the difference between said first and second total phases; optical detection means, coupled to said combining means, for converting said at least one recombined beam into at least one electrical signal representing the optical power of said at least one recombined beam; and third means, responsive to said at least one electrical signal, for providing an output signal that depends on the amount of analyte bound to said active region based on said phase change undergone by said at least one of said first and second beams encountering said active and inactive regions.
申请日期	1994-06-24
专利号	AU1994073172A1
专利状态	失效
申请号	AU1994073172
公开（公告）号	AU1994073172A1
IPC 分类号	G01N21/45 \| G01N21/75 \| G01J3/45 \| G01N21/00 \| G01N21/01 \| G01N21/41 \| G01N33/53 \| G01N33/545 \| G01N33/551 \| G01N33/552 \| G01N33/566 \| G05B1/06 \| G05B11/18 \| H01S3/13
专利代理人	-
代理机构	-
文献类型	专利
条目标识符	http://ir.opt.ac.cn/handle/181661/62439
专题	半导体激光器专利数据库
作者单位	FIRST MEDICAL, INC.
推荐引用方式 GB/T 7714	ALLEN, JIMMY D.,COBB, MICHAEL E.,GUSTAFSON, ERIC K. Solid-phase binding assay system and method for interferometrically measuring analytes bound to an active receptor. AU1994073172A1[P]. 1995-01-24.