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Axial resolution enhancement of light-sheet microscopy by double scanning of Bessel beam and its complementary beam
Jia, Hao1,2; Yu, Xianghua1; Yang, Yanlong1; Zhou, Xing1,2; Yan, Shaohui1; Liu, Chao1,2; Lei, Ming1; Yao, Baoli1
Department瞬态光学技术国家重点实验室
2019-01
Source PublicationJOURNAL OF BIOPHOTONICS
ISSN1864-063X;1864-0648
Volume12Issue:1
Contribution Rank1
Abstract

The side lobes of Bessel beam will create significant out-of-focus background when scanned in light-sheet fluorescence microscopy (LSFM), limiting the axial resolution of the imaging system. Here, we propose to overcome this issue by scanning the sample twice with zeroth-order Bessel beam and another type of propagation-invariant beam, complementary to the zeroth-order Bessel beam, which greatly reduces the out-of-focus background created in the first scan. The axial resolution can be improved from 1.68 mu m of the Bessel light-sheet to 1.07 mu m by subtraction of the two scanned images across a whole field-of-view of up to 300 mu m x 200 mu m x 200 mu m. The optimization procedure to create the complementary beam is described in detail and it is experimentally generated with a spatial light modulator. The imaging performance is validated experimentally with fluorescent beads as well as eGFP-labeled mouse brain neurons.

KeywordBessel beam laser beam shaping light-sheet fluorescence microscopy three-dimensional microscopy
DOI10.1002/jbio.201800094
Indexed BySCI
Language英语
WOS IDWOS:000455115500018
PublisherWILEY-V C H VERLAG GMBH
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.opt.ac.cn/handle/181661/31181
Collection瞬态光学技术国家重点实验室
Corresponding AuthorYao, Baoli
Affiliation1.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian 710119, Shaanxi, Peoples R China
2.Univ Chinese Acad Sci, Sch Mat, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Jia, Hao,Yu, Xianghua,Yang, Yanlong,et al. Axial resolution enhancement of light-sheet microscopy by double scanning of Bessel beam and its complementary beam[J]. JOURNAL OF BIOPHOTONICS,2019,12(1).
APA Jia, Hao.,Yu, Xianghua.,Yang, Yanlong.,Zhou, Xing.,Yan, Shaohui.,...&Yao, Baoli.(2019).Axial resolution enhancement of light-sheet microscopy by double scanning of Bessel beam and its complementary beam.JOURNAL OF BIOPHOTONICS,12(1).
MLA Jia, Hao,et al."Axial resolution enhancement of light-sheet microscopy by double scanning of Bessel beam and its complementary beam".JOURNAL OF BIOPHOTONICS 12.1(2019).
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