OPT OpenIR  > 瞬态光学技术国家重点实验室
Axial resolution enhancement of light-sheet microscopy by double scanning of Bessel beam and its complementary beam
Jia, Hao1,2; Yu, Xianghua1; Yang, Yanlong1; Zhou, Xing1,2; Yan, Shaohui1; Liu, Chao1,2; Lei, Ming1; Yao, Baoli1
作者部门瞬态光学技术国家重点实验室
2019-01
发表期刊JOURNAL OF BIOPHOTONICS
ISSN1864-063X;1864-0648
卷号12期号:1
产权排序1
摘要

The side lobes of Bessel beam will create significant out-of-focus background when scanned in light-sheet fluorescence microscopy (LSFM), limiting the axial resolution of the imaging system. Here, we propose to overcome this issue by scanning the sample twice with zeroth-order Bessel beam and another type of propagation-invariant beam, complementary to the zeroth-order Bessel beam, which greatly reduces the out-of-focus background created in the first scan. The axial resolution can be improved from 1.68 mu m of the Bessel light-sheet to 1.07 mu m by subtraction of the two scanned images across a whole field-of-view of up to 300 mu m x 200 mu m x 200 mu m. The optimization procedure to create the complementary beam is described in detail and it is experimentally generated with a spatial light modulator. The imaging performance is validated experimentally with fluorescent beads as well as eGFP-labeled mouse brain neurons.

关键词Bessel beam laser beam shaping light-sheet fluorescence microscopy three-dimensional microscopy
DOI10.1002/jbio.201800094
收录类别SCI
语种英语
WOS记录号WOS:000455115500018
出版者WILEY-V C H VERLAG GMBH
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.opt.ac.cn/handle/181661/31181
专题瞬态光学技术国家重点实验室
通讯作者Yao, Baoli
作者单位1.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian 710119, Shaanxi, Peoples R China
2.Univ Chinese Acad Sci, Sch Mat, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Jia, Hao,Yu, Xianghua,Yang, Yanlong,et al. Axial resolution enhancement of light-sheet microscopy by double scanning of Bessel beam and its complementary beam[J]. JOURNAL OF BIOPHOTONICS,2019,12(1).
APA Jia, Hao.,Yu, Xianghua.,Yang, Yanlong.,Zhou, Xing.,Yan, Shaohui.,...&Yao, Baoli.(2019).Axial resolution enhancement of light-sheet microscopy by double scanning of Bessel beam and its complementary beam.JOURNAL OF BIOPHOTONICS,12(1).
MLA Jia, Hao,et al."Axial resolution enhancement of light-sheet microscopy by double scanning of Bessel beam and its complementary beam".JOURNAL OF BIOPHOTONICS 12.1(2019).
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Axial resolution enh(2113KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Jia, Hao]的文章
[Yu, Xianghua]的文章
[Yang, Yanlong]的文章
百度学术
百度学术中相似的文章
[Jia, Hao]的文章
[Yu, Xianghua]的文章
[Yang, Yanlong]的文章
必应学术
必应学术中相似的文章
[Jia, Hao]的文章
[Yu, Xianghua]的文章
[Yang, Yanlong]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Axial resolution enhancement of light-sheet microscopy by double scanning of Bessel beam and its complementary beam.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。