OPT OpenIR  > 信息光子学研究室
Optofluidic twin-core hollow fiber interferometer for label-free sensing of the streptavidin-biotin binding
Yang, Xinghua1; Yu, Wenting1; Liu, Zhihai1; Yang, Jun1; Zhang, Yu1; Kong, Depeng2; Long, Qunlong1; Yuan, Tingting1; Cao, Jiaming1; Yuan, Libo1,3; Oh, Kyunghwan4
Department信息光子学研究室
2018-12-20
Source PublicationSensors and Actuators, B: Chemical
ISSN09254005
Volume277Pages:353-359
Contribution Rank2
Abstract

We proposed and experimentally demonstrated an optofluidic biosensor using a twin-core hollow optical fiber (TC-HOF) interferometer for label-free sensing of the streptavidin-biotin binding. The TC-HOF had a central hole and two cores were imbedded near its circumference. The central hole functioned as a microfluidic channel where the liquid was flown. One of the cores was suspended at the inner surface of the hole and served as sensing arm while the other core embedded in the cladding as the reference arm, which formed an integrated all-fiber Mach-Zehnder interferometer (MZI). Both ends of TC-HOF were serially spliced to multimode fiber-single mode fiber assembly, whose lateral positions were adjusted to maximize the interference visibility. Before the surface functionalization, the pristine TC-HOF MZI showed the RI sensitivity of 2577 nm/RIU. The suspended core's surface was chemically modified with streptavidin and biotin solutions were injected through micro-HOles in TC-HOF. Through the specific binding reaction between biotin and streptavidin, the effective refractive index (RI) around the sensing arm was modulated, which was detected by the spectral shifts in the interference patterns. The proposed sensor showed an excellent linear response to the biotin concentration in the range of 0.01–0.1 mg/mL and the net detection sensitivity of biotin was 16.9 nm/(mg/mL), which was achieved in a label-free manner. This device can be further applied to sensing and monitoring of the biochemical binding of biotin with various biomolecules such as cells, antigens, antibodies, and DNA in real-time and in situ. © 2018

DOI10.1016/j.snb.2018.09.032
Indexed BySCI ; EI
Language英语
WOS IDWOS:000453066700044
PublisherElsevier B.V.
EI Accession Number20183805825578
Citation statistics
Document Type期刊论文
Identifierhttp://ir.opt.ac.cn/handle/181661/30652
Collection信息光子学研究室
Corresponding AuthorYang, Xinghua
Affiliation1.Key Laboratory of In-Fiber Integrated Optics, Ministry of Education, College of Science, Harbin Engineering University, Harbin; 150001, China;
2.State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanic, Chinese Academy of Sciences, Xi'an; 710119, China;
3.Photonics Research Center, Guilin University of Electronics Technology, Guilin; 541004, China;
4.Department of Physics, Yonsei University, Seoul; 03722, Korea, Republic of
Recommended Citation
GB/T 7714
Yang, Xinghua,Yu, Wenting,Liu, Zhihai,et al. Optofluidic twin-core hollow fiber interferometer for label-free sensing of the streptavidin-biotin binding[J]. Sensors and Actuators, B: Chemical,2018,277:353-359.
APA Yang, Xinghua.,Yu, Wenting.,Liu, Zhihai.,Yang, Jun.,Zhang, Yu.,...&Oh, Kyunghwan.(2018).Optofluidic twin-core hollow fiber interferometer for label-free sensing of the streptavidin-biotin binding.Sensors and Actuators, B: Chemical,277,353-359.
MLA Yang, Xinghua,et al."Optofluidic twin-core hollow fiber interferometer for label-free sensing of the streptavidin-biotin binding".Sensors and Actuators, B: Chemical 277(2018):353-359.
Files in This Item:
File Name/Size DocType Version Access License
Optofluidic twin-cor(2481KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yang, Xinghua]'s Articles
[Yu, Wenting]'s Articles
[Liu, Zhihai]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yang, Xinghua]'s Articles
[Yu, Wenting]'s Articles
[Liu, Zhihai]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yang, Xinghua]'s Articles
[Yu, Wenting]'s Articles
[Liu, Zhihai]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Optofluidic twin-core hollow fiber interferometer for label-free sensing of the streptavidin-biotin binding.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.