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Single-shot time-gated fluorescence lifetime imaging using three-frame images
Li, Yahui1,2,3; Jia, Hui3; Chen, Shaorong3; Tian, Jinshou1,2; Liang, Lingliang3; Yuan, Fenfang3; Yu, Hongqi3; Li, David Day-Uei4; Li, David Day-Uei (david.li@strath.ac.uk)
Department精密物理量测量实验室
2018-07-09
Source PublicationOptics Express
ISSN10944087
Volume26Issue:14Pages:17936-17947
Contribution Rank1
Abstract

Qualitative and quantitative measurements of complex flows demand for fast single-shot fluorescence lifetime imaging (FLI) technology with high precision. A method, single-shot time-gated fluorescence lifetime imaging using three-frame images (TFI-TGFLI), is presented. To our knowledge, it is the first work to combine a three-gate rapid lifetime determination (RLD) scheme and a four-channel framing camera to achieve this goal. Di erent from previously proposed two-gate RLD schemes, TFI-TGFLI can provide a wider lifetime range 0.6 ∼ 13ns with reasonable precision. The performances of the proposed approach have been examined by both Monte-Carlo simulations and toluene seeded gas mixing jet diagnosis experiments. The measured average lifetimes of the whole excited areas agree well with the results obtained by the streak camera, and they are 7.6ns (N2= 7L/min; O22= 19L/min; O2= 1L/min) with the standard deviations of 1.7ns and 0.8ns among the lifetime image pixels, respectively. The concentration distributions of the quenchers and fluorescent species were further analyzed, and they are consistent with the experimental settings. © 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.

DOI10.1364/OE.26.017936
Indexed BySCIE ; EI
Language英语
WOS SubjectOptics
WOS IDWOS:000438209100027
EI Accession Number20182805532440
EI Classification NumberComputers And Data Processing::Computer Software, Data hAndling And Applications::Artificial Intelligence
Citation statistics
Document Type期刊论文
Identifierhttp://ir.opt.ac.cn/handle/181661/30450
Collection精密物理量测量实验室
Corresponding AuthorLi, David Day-Uei (david.li@strath.ac.uk)
Affiliation1.Key Laboratory of Ultra-Fast Photoelectric Diagnostics Technology, Xian Institute of Optics and Precision Mechanics, Xian, Shanxi; 710049, China
2.University of Chinese Academy of Sciences, Beijing; 100049, China
3.Laboratory of Ultra-Fast Imaging, College of Electronic Science and Engineering, National University of Defense Technology, Changsha, Hunan; 410073, China
4.Faculty of Science, University of Strathclyde, G4 0RE, Glasgow Scotland, United Kingdom
Recommended Citation
GB/T 7714
Li, Yahui,Jia, Hui,Chen, Shaorong,et al. Single-shot time-gated fluorescence lifetime imaging using three-frame images[J]. Optics Express,2018,26(14):17936-17947.
APA Li, Yahui.,Jia, Hui.,Chen, Shaorong.,Tian, Jinshou.,Liang, Lingliang.,...&Li, David Day-Uei .(2018).Single-shot time-gated fluorescence lifetime imaging using three-frame images.Optics Express,26(14),17936-17947.
MLA Li, Yahui,et al."Single-shot time-gated fluorescence lifetime imaging using three-frame images".Optics Express 26.14(2018):17936-17947.
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