| Two-Photon Laser Scanning Stereomicroscopy for Fast Volumetric Imaging | |
| Yang, Yanlong1; Yao, Baoli1; Lei, Ming1; Dan, Dan1; Li, Runze1; Van Horn, Mark2; Chen, Xun3,4; Li, Yang3,4; Ye, Tong3,4,5; Yao, BL (reprint author), Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian, Peoples R China. | |
| 作者部门 | 瞬态光学技术国家重点实验室 |
| 2016-12-20 | |
| 发表期刊 | PLOS ONE
 |
| ISSN | 1932-6203 |
| 卷号 | 11期号:12 |
| 产权排序 | 1 |
| 摘要 | Bessel beams have been successfully used in two-photon laser scanning fluorescence microscopy to extend the depth of field (EDF), which makes it possible to observe fast events volumetrically. However, the depth information is lost due to integration of fluorescence signals along the propagation direction. We describe the design and implementation of two-photon lasers scanning stereomicroscopy, which allows viewing dynamic processes in three-dimensional (3D) space stereoscopically in real-time with shutter glasses at the speed of 1.4 volumes per second. The depth information can be appreciated by human visual system or be recovered with correspondence algorithms for some cases. |
| 文章类型 | Article |
| 学科领域 | Multidisciplinary Sciences |
| WOS标题词 | Science & Technology |
| DOI | 10.1371/journal.pone.0168885 |
| 收录类别 | SCI |
| 关键词[WOS] | LIGHT-SHEET MICROSCOPY ; FLUORESCENCE MICROSCOPY ; DEEP |
| 语种 | 英语 |
| WOS研究方向 | Science & Technology - Other Topics |
| 项目资助者 | National Institute of General Medical Sciences(R21GM104683 ; National Science Foundation(1539034) ; National Natural Science Foundation of China (NSFC)(81427802 ; P20GM103499) ; 61275191 ; 61522511) |
| WOS类目 | Multidisciplinary Sciences |
| WOS记录号 | WOS:000392842900083 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.opt.ac.cn/handle/181661/28666 |
| 专题 | 瞬态光学研究室 |
| 通讯作者 | Yao, BL (reprint author), Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian, Peoples R China 2.Med Univ South Carolina, Dept Radiol, Charleston, SC USA 3.Clemson Univ, Dept Bioengn, Clemson, SC 29634 USA 4.Clemson Univ, COMSET, Clemson, SC 29634 USA 5.Med Univ South Carolina, Dept Regenerat Med & Cell Biol, Charleston, SC 29425 USA |
| 推荐引用方式 GB/T 7714 | Yang, Yanlong,Yao, Baoli,Lei, Ming,et al. Two-Photon Laser Scanning Stereomicroscopy for Fast Volumetric Imaging[J]. PLOS ONE,2016,11(12). |
| APA | Yang, Yanlong.,Yao, Baoli.,Lei, Ming.,Dan, Dan.,Li, Runze.,...&Yao, BL .(2016).Two-Photon Laser Scanning Stereomicroscopy for Fast Volumetric Imaging.PLOS ONE,11(12). |
| MLA | Yang, Yanlong,et al."Two-Photon Laser Scanning Stereomicroscopy for Fast Volumetric Imaging".PLOS ONE 11.12(2016). |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Two-Photon Laser Sca（3224KB） | 期刊论文 | 作者接受稿 | 限制开放 | CC BY-NC-SA | 请求全文 | |
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