| The pH-dependence of photochemical intermediates of O and P in bacteriorhodopsin by continuous light | |
| Wang, LP; Shen, ZS; Wang, JW; Li, BF; Chen, F; Yang, WZ; Feng, XQ | |
| 2006-05-12 | |
| 发表期刊 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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| 卷号 | 343期号:3页码:899-903 |
| 摘要 | The pH-dependence of the O and P intermediates in the photocycle of bacteriorhodopsin (bR) on the intensity and duration of the exciting flash was investigated for bR glycerol suspensions and bR gelatin films. Green and red laser flashes (532 and 670 nm) were utilized to generate a photoequilibrium state of bR and O at ambient temperature, and UV-vis spectroscopy was used to determine the photoconversion for the bR suspensions and films. The maximal concentration of the O intermediate was observed to be pH-dependent and the dependency was most pronounced at a slightly alkaline pH values. The photochemical conversion from the O to P intermediate was investieated for both bR suspensions and films. The P intermediate was only found in bR gelatin film. These results indicate that bR gelatin film may be an attractive candidate for the information storage based on P intermediate. It is possible, with red light, to create photoproducts which are thermally stable at ambient temperature and that can be photochemically erased. (c) 2006 Elsevier Inc. All rights reserved. |
| 文章类型 | Article |
| 关键词 | Bacteriorhodopsin Photoequilibrium State Ph-dependence o Intermediate Photocycle Laser |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1016/j.bbrc.2006.03.041 |
| 收录类别 | SCI |
| 关键词[WOS] | PROTEIN RESIDUES ; PHOTOCYCLE ; RELEASE ; PROTONATION ; ASP-85 ; O-630 ; MODEL ; PUMP |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| WOS记录号 | WOS:000236976800031 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.opt.ac.cn/handle/181661/25182 |
| 专题 | 瞬态光学研究室 |
| 作者单位 | 1.Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China 2.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China 3.Chinese Acad Sci, Xian Inst Opt & Fine Mech, Xian 710068, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, LP,Shen, ZS,Wang, JW,et al. The pH-dependence of photochemical intermediates of O and P in bacteriorhodopsin by continuous light[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2006,343(3):899-903. |
| APA | Wang, LP.,Shen, ZS.,Wang, JW.,Li, BF.,Chen, F.,...&Feng, XQ.(2006).The pH-dependence of photochemical intermediates of O and P in bacteriorhodopsin by continuous light.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,343(3),899-903. |
| MLA | Wang, LP,et al."The pH-dependence of photochemical intermediates of O and P in bacteriorhodopsin by continuous light".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 343.3(2006):899-903. |
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| The pH-dependence of（154KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 请求全文 | |
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