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Quantitative Phase Contrast Microscopy with Optimized Partially Coherent Illumination
Zhuo, Kequn1,2,3; Wang, Yang1,3; Ma, Ying1,3; An, Sha1,3; Zalevsky, Zeev4; Zheng, Juanjuan1,3; Gao, Peng1,3
Department瞬态光学研究室
2023-04
Source PublicationPHOTONICS
ISSN2304-6732
Volume10Issue:4
Contribution Rank1
Abstract

This paper presents a partially coherent illumination quantitative phase contrast microscopic (PCI-QPCM) prototype. In the PCI-QPCM prototype, the light scattered by a rotating diffuser is coupled into a multi-mode fiber, and the output light is used as the illumination for PCI-QPCM. The illumination wave has a constrained spectrum with a diameter of tens of micrometers, which can reduce speckle noise and will not broaden the dc term of the object wave. In the Fourier plane of the object wave, grating-masked phase shifters generated by a spatial light modulator (SLM) allow for measuring the intensity of the undiffracted and diffracted components of the object wave, as well as the phase-shifted interference patterns of the two. Quantitative phase images can be reconstructed from the recorded intensity images. The proposed PCI-QPCM was demonstrated with quantitative phase imaging of a transparent waveguide and a phase-step sample.

Keywordquantitative phase contrast microscopy (QPCM) partially coherent illumination spatial light modulator (SLM)
DOI10.3390/photonics10040391
Indexed BySCI
Language英语
WOS IDWOS:000979542500001
PublisherMDPI
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.opt.ac.cn/handle/181661/96451
Collection瞬态光学研究室
Corresponding AuthorZheng, Juanjuan; Gao, Peng
Affiliation1.Xidian Univ, Sch Phys & Optoelect Engn, Xian 710071, Peoples R China
2.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian 710019, Peoples R China
3.Univ Shaanxi Prov, Engn Res Ctr Informat Nanomat, Xian 710071, Peoples R China
4.Bar Ilan Univ, Fac Engn, Nano Technol Ctr, IL-5290002 Ramat Gan, Israel
Recommended Citation
GB/T 7714
Zhuo, Kequn,Wang, Yang,Ma, Ying,et al. Quantitative Phase Contrast Microscopy with Optimized Partially Coherent Illumination[J]. PHOTONICS,2023,10(4).
APA Zhuo, Kequn.,Wang, Yang.,Ma, Ying.,An, Sha.,Zalevsky, Zeev.,...&Gao, Peng.(2023).Quantitative Phase Contrast Microscopy with Optimized Partially Coherent Illumination.PHOTONICS,10(4).
MLA Zhuo, Kequn,et al."Quantitative Phase Contrast Microscopy with Optimized Partially Coherent Illumination".PHOTONICS 10.4(2023).
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