| Super-resolution multicolor fluorescence microscopy enabled by an apochromatic super-oscillatory lens with extended depth-of-focus | |
Li, Wenli1,2,3; He, Pei1,2,3; Fan, Yulong4; Du, Yangtao5; Gao, Bo6 ; Chu, Zhiqin7; An, Chengxu1,2,3; Lei, Dangyuan4; Yuan, Weizheng1,2,3; Yu, Yiting1,2,3
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| 作者部门 | 光谱成像技术研究室 |
| 2022-06-05 | |
| 出处 | arXiv |
| ISSN | 23318422 |
| 产权排序 | 6 |
| 摘要 | Multicolor super-resolution imaging remains an intractable challenge for both far-field and near-field based super-resolution techniques. Planar super-oscillatory lens (SOL), a far-field subwavelength-focusing diffractive lens device, holds great potential for achieving sub-diffraction-limit imaging at multiple wavelengths. However, conventional SOL devices suffer from a numerical aperture (NA) related intrinsic tradeoff among the depth of focus (DoF), chromatic dispersion and focus spot size, being an essential characteristics of common diffractive optical elements. Typically, the limited DoF and significant chromatism associated with high NA can lead to unfavorable degradation of image quality although increasing NA imporves the resolution. Here, we apply a multi-objective genetic algorithm (GA) optimization approach to design an apochromatic binary-phase SOL that generates axially jointed multifoci concurrently having prolonged DoF, customized working distance (WD) and suppressed side-lobes yet minimized main-lobe size, optimizing the aforementioned NA-dependent tradeoff. Experimental implementation of this GA-optimized SOL demonstrates simultaneous focusing of blue, green and red light beams into an optical needle of ∼0.5λ in diameter and >10λ in length (DoF) at 428 μm WD, resulting in an ultimate resolution better than λ/3 in the lateral dimension. By integrating this apochromatic SOL device with a commercial fluorescence microscope, we employ the optical needle to perform, for the first time, three-dimensional super-resolution multicolor fluorescence imaging of the "unseen" fine structure of neurons at one go. The present study provides not only a practical route to far-field multicolor super-resolution imaging but also a viable approach for constructing imaging systems avoiding complex sample positioning and unfavorable photobleaching. Copyright © 2022, The Authors. All rights reserved. |
| 收录类别 | EI |
| 语种 | 英语 |
| 出版者 | arXiv |
| EI入藏号 | 20220153951 |
| 文献类型 | 预印本 |
| 条目标识符 | http://ir.opt.ac.cn/handle/181661/96045 |
| 专题 | 光谱成像技术研究室 |
| 作者单位 | 1.Research & Development Institute of Northwestern Polytechnical University in Shenzhen, College of Mechanical Engineering, Ningbo Institute of Northwestern Polytechnical University, Northwestern Polytechnical University, Xi'An; 710072, China; 2.Key Laboratory of Micro/Nano Systems for Aerospace (Ministry of Education), Northwestern Polytechnical University, Xi'An; 710072, China; 3.Shaanxi Province Key Laboratory of Micro and Nano Electro-Mechanical Systems, Northwestern Polytechnical University, Xi'An; 710072, China; 4.Department of Materials Science and Engineering, City University of Hong Kong, 999077, Hong Kong; 5.The Institute of Ai and Robotics, Fudan University, Shanghai; 200433, China; 6.Key Laboratory of Spectral Imaging Technology of Chinese Academy of Sciences, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xian; 710119, China; 7.Department of Electrical and Electronic Engineering, Joint Appointment with School of Biomedical Sciences, The University of Hong Kong, 999077, Hong Kong |
| 推荐引用方式 GB/T 7714 | Li, Wenli,He, Pei,Fan, Yulong,et al. Super-resolution multicolor fluorescence microscopy enabled by an apochromatic super-oscillatory lens with extended depth-of-focus. 2022. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Super-resolution mul（1451KB） | 预印本 | 限制开放 | CC BY-NC-SA | 请求全文 | ||
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