High Spatio-Temporal Resolution Condenser-Free Quantitative Phase Contrast Microscopy

doi:10.3389/fphy.2022.892529

OPT OpenIR > 瞬态光学研究室

	High Spatio-Temporal Resolution Condenser-Free Quantitative Phase Contrast Microscopy
	Ma, Ying; Ma, Lin; Zheng, Juanjuan; Liu, Min; Zalevsky, Zeev; Gao, Peng
作者部门	瞬态光学研究室
	2022-05-09
发表期刊	FRONTIERS IN PHYSICS
ISSN	2296-424X
卷号	10
产权排序	3
摘要	Quantitative phase-contrast microscopy (QPCM) provides an effective approach for label-free detection of transparent samples. In this study, we propose a condenser-free quantitative phase-contrast microscopy (CF-QPCM), in which several light-emitting diodes (LEDs) distributed on a ring are used for direct ultra-oblique illumination. Such condenser-free design greatly simplifies the system's structure and releases the space for installing samples. Quantitative phase maps are reconstructed by retarding the unscattered components of the object waves for a series of phases 0, pi/2, pi, and 3 pi/2 through a high-speed spatial light modulator (SLM). With this system, quantitative phase imaging of live cells has been achieved at a spatial resolution of 231 nm (lateral) and a frame rate of 250 Hz. We believe that the proposed CF-QPCM can contribute to biomedical, industrial, chemistry fields, etc.
关键词	quantitative phase imaging phase contrast ultra-oblique illumination label-free non-invasive
DOI	10.3389/fphy.2022.892529
收录类别	SCI
语种	英语
WOS记录号	WOS:000803908000001
出版者	FRONTIERS MEDIA SA
引用统计
文献类型	期刊论文
条目标识符	http://ir.opt.ac.cn/handle/181661/95997
专题	瞬态光学研究室
推荐引用方式 GB/T 7714	Ma, Ying,Ma, Lin,Zheng, Juanjuan,et al. High Spatio-Temporal Resolution Condenser-Free Quantitative Phase Contrast Microscopy[J]. FRONTIERS IN PHYSICS,2022,10.
APA	Ma, Ying,Ma, Lin,Zheng, Juanjuan,Liu, Min,Zalevsky, Zeev,&Gao, Peng.(2022).High Spatio-Temporal Resolution Condenser-Free Quantitative Phase Contrast Microscopy.FRONTIERS IN PHYSICS,10.
MLA	Ma, Ying,et al."High Spatio-Temporal Resolution Condenser-Free Quantitative Phase Contrast Microscopy".FRONTIERS IN PHYSICS 10(2022).

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
High Spatio-Temporal（2702KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	请求全文