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Large-field structured illumination microscopy based on 2D grating and a spatial light modulator
Wen, Kai1,2; Fang, Xiang1; Ma, Ying1; Liu, Min1; An, Sha1; Zheng, JuanJuan1,3; Kozacki, Tomasz2; Gao, Peng1
Department瞬态光学研究室
2022-06-01
Source PublicationOptics Letters
ISSN01469592;15394794
Volume47Issue:11Pages:2666-2669
Contribution Rank3
Abstract

Structured illumination microscopy (SIM) has been widely used in biological research due to its merits of fast imaging speed, minimal invasiveness, super-resolution, and optical sectioning imaging capability. However, the conventional SIM that uses a spatial light modulator (SLM) for fringe projection often has a limited imaging field of view. Herein, we report a large-field SIM technique that combines a 2D grating for fringe pattern projection and an SLM for selecting fringe orientation and performing phase shifting digitally. The proposed SIM technique breaks the bottleneck of fringe number limited by the digital projection devices, while maintaining the advantage of high-speed (digital) phase shifting of conventional SIM. The method avoids the pixilation and dispersion effects of the SLMs. Finally, a 1.8-fold resolution enhancement in a large field of 690 × 517 µm2 under a 20×/NA0.75 objective is experimentally demonstrated. The proposed technique can be widely applied to biology, chemistry, and industry. © 2022 Optica Publishing Group

DOI10.1364/OL.460292
Indexed BySCI ; EI
Language英语
WOS IDWOS:000807404900015
PublisherOptica Publishing Group (formerly OSA)
EI Accession Number20222212182743
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.opt.ac.cn/handle/181661/95986
Collection瞬态光学研究室
Corresponding AuthorGao, Peng
Affiliation1.School of Physics and Optoelectronic Engineering, Xidian University, Xi’an; 710071, China;
2.Department of Mechatronics, Institute of Micromechanics and Photonics, Warsaw University of Technology, Warsaw; 02-525, Poland;
3.State Key Laboratory of Transient Optics and Photonics, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an; 710119, China
Recommended Citation
GB/T 7714
Wen, Kai,Fang, Xiang,Ma, Ying,et al. Large-field structured illumination microscopy based on 2D grating and a spatial light modulator[J]. Optics Letters,2022,47(11):2666-2669.
APA Wen, Kai.,Fang, Xiang.,Ma, Ying.,Liu, Min.,An, Sha.,...&Gao, Peng.(2022).Large-field structured illumination microscopy based on 2D grating and a spatial light modulator.Optics Letters,47(11),2666-2669.
MLA Wen, Kai,et al."Large-field structured illumination microscopy based on 2D grating and a spatial light modulator".Optics Letters 47.11(2022):2666-2669.
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