Xi'an Institute of Optics and Precision Mechanics,CAS
| Histogram clustering for rapid time-domain fluorescence lifetime image analysis | |
Li, Yahui1,2; Sapermsap, Natakorn3; Yu, Jun4; Tian, Jinshou1,2 ; Chen, Yu3; Li, David Day-Uei5
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| 作者部门 | 条纹相机工程中心 |
| 2021-07-01 | |
| 发表期刊 | Biomedical Optics Express
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| ISSN | 21567085 |
| 卷号 | 12期号:7页码:4293-4307 |
| 产权排序 | 1 |
| 摘要 | We propose a histogram clustering (HC) method to accelerate fluorescence lifetime imaging (FLIM) analysis in pixel-wise and global fitting modes. The proposed method's principle was demonstrated, and the combinations of HC with traditional FLIM analysis were explained. We assessed HC methods with both simulated and experimental datasets. The results reveal that HC not only increases analysis speed (up to 106 times) but also enhances lifetime estimation accuracy. Fast lifetime analysis strategies were suggested with execution times around or below 30 µs per histograms on MATLAB R2016a, 64-bit with the Intel Celeron CPU (2950M @ 2GHz). © 2021 OSA - The Optical Society. All rights reserved. |
| DOI | 10.1364/BOE.427532 |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000670305800004 |
| 出版者 | The Optical Society |
| EI入藏号 | 20212610574504 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.opt.ac.cn/handle/181661/94938 |
| 专题 | 条纹相机工程中心 |
| 作者单位 | 1.Key Laboratory of Ultra-fast Photoelectric Diagnostics Technology, Xi'an Institute of Optics and Precision Mechanics, Shaanxi, Xi'an; 710049, China; 2.Collaborative Innovation Center of Extreme Optics, Shanxi University, Shanxi, Taiyuan; 030006, China; 3.Department of Physics, Scottish Universities Physics Alliance, University of Strathclyde, Glasgow; G4 0NG, United Kingdom; 4.Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow; G4 0RE, United Kingdom; 5.Department of Biomedical Engineering, University of Strathclyde, Glasgow; G1 0NW, United Kingdom |
| 推荐引用方式 GB/T 7714 | Li, Yahui,Sapermsap, Natakorn,Yu, Jun,et al. Histogram clustering for rapid time-domain fluorescence lifetime image analysis[J]. Biomedical Optics Express,2021,12(7):4293-4307. |
| APA | Li, Yahui,Sapermsap, Natakorn,Yu, Jun,Tian, Jinshou,Chen, Yu,&Li, David Day-Uei.(2021).Histogram clustering for rapid time-domain fluorescence lifetime image analysis.Biomedical Optics Express,12(7),4293-4307. |
| MLA | Li, Yahui,et al."Histogram clustering for rapid time-domain fluorescence lifetime image analysis".Biomedical Optics Express 12.7(2021):4293-4307. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Histogram clustering（8261KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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