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Investigations on Average Fluorescence Lifetimes for Visualizing Multi-Exponential Decays
Li, Yahui1,2,3; Natakorn, Sapermsap4; Chen, Yu4; Safar, Mohammed1; Cunningham, Margaret1; Tian, Jinshou2,3; Li, David Day-Uei1
作者部门条纹相机工程中心
2020-10-16
发表期刊FRONTIERS IN PHYSICS
ISSN2296-424X
卷号8
产权排序1
摘要

Intensity- and amplitude-weighted average lifetimes, denoted as tau(I) and tau(A) hereafter, are useful indicators for revealing Forster resonance energy transfer (FRET) or fluorescence quenching behaviors. In this work, we discussed the differences between tau(I) and tau(A) and presented several model-free lifetime determination algorithms (LDA), including the center-of-mass, phasor, and integral equation methods for fast tau(I) and tau(A) estimations. For model-based LDAs, we discussed the model-mismatch problems, and the results suggest that a bi-exponential model can well approximate a signal following a multi-exponential model. Depending on the application requirements, suggestions about the LDAs to be used are given. The instrument responses of the imaging systems were included in the analysis. We explained why only using the tau(I) model for FRET analysis can be misleading; both tau(I) and tau(A) models should be considered. We also proposed using tau(A)/tau(I) as a new indicator on two-photon fluorescence lifetime images, and the results show that tau(A)/tau(I) is an intuitive tool for visualizing multi-exponential decays.

关键词fluorescence lifetime imaging lifetime determination algorithm average lifetimes multi-exponential decays lifetime image visualization FRET— fluorescence resonance energy transfer
DOI10.3389/fphy.2020.576862
收录类别SCI
语种英语
WOS记录号WOS:000584706400001
出版者FRONTIERS MEDIA SA
引用统计
被引频次:29[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.opt.ac.cn/handle/181661/93785
专题条纹相机工程中心
通讯作者Li, David Day-Uei
作者单位1.Univ Strathclyde, Strathcyde Inst Pharm & Biomed Sci, Glasgow, Lanark, Scotland
2.Xian Inst Opt & Precis Mech, Key Lab Ultrafast Photoelect Diagnost Technol, Xian, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Univ Strathcyde, Scottish Univ Phys Alliance, Dept Phys, Glasgow, Lanark, Scotland
推荐引用方式
GB/T 7714
Li, Yahui,Natakorn, Sapermsap,Chen, Yu,et al. Investigations on Average Fluorescence Lifetimes for Visualizing Multi-Exponential Decays[J]. FRONTIERS IN PHYSICS,2020,8.
APA Li, Yahui.,Natakorn, Sapermsap.,Chen, Yu.,Safar, Mohammed.,Cunningham, Margaret.,...&Li, David Day-Uei.(2020).Investigations on Average Fluorescence Lifetimes for Visualizing Multi-Exponential Decays.FRONTIERS IN PHYSICS,8.
MLA Li, Yahui,et al."Investigations on Average Fluorescence Lifetimes for Visualizing Multi-Exponential Decays".FRONTIERS IN PHYSICS 8(2020).
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