OPT OpenIR  > 条纹相机工程中心
A rapid analysis platform for investigating the cellular locations of bacteria using two-photon fluorescence lifetime imaging microscopy
Sapermsap, Natakorn1; Li, David Day-Uei2; Al-Hemedawi, Ryath2; Li, Yahui2,3; Yu, Jun2; Birch, David J. S.1; Chen, Yu1
作者部门条纹相机工程中心
2020-07
发表期刊METHODS AND APPLICATIONS IN FLUORESCENCE
ISSN2050-6120
卷号8期号:3
产权排序3
摘要

Facultative intracellular pathogens are able to live inside and outside host cells. It is highly desirable to differentiate their cellular locations for the purposes of fundamental research and clinical applications. In this work, we developed a novel analysis platform that allows users to choose two analysis models: amplitude weighted lifetime (tau(A)) and intensity weighted lifetime (tau(I)) for fluorescence lifetime imaging microscopy (FLIM). We applied these two models to analyse FLIM images of mouse Raw macrophage cells that were infected with bacteria Shigella Sonnei, adherent and invasive E. coli (AIEC) and Lactobacillus. The results show that the fluorescence lifetimes of bacteria depend on their cellular locations. The tau(A) model is superior in visually differentiating bacteria that are in extra- and intra-cellular and membrane-bounded locations, whereas the tau(I) model show excellent precision. Both models show speedy performances that analysis can be performed within 0.3 s. We also compared the proposed models with a widely used commercial software tool (tau(C), SPC Image, Becker & Hickl GmbH), showing similar tau(I) and tau(C) results. The platform also allows users to perform phasor analysis with great flexibility to pinpoint the regions of interest from lifetime images as well as phasor plots. This platform holds the disruptive potential of replacing z-stack imaging for identifying intracellular bacteria.

关键词fluorescence lifetime FLIM two-photon excitation
DOI10.1088/2050-6120/ab854e
收录类别SCI
语种英语
WOS记录号WOS:000528572700001
出版者IOP PUBLISHING LTD
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.opt.ac.cn/handle/181661/93396
专题条纹相机工程中心
作者单位1.Univ Strathclyde, Dept Phys, SUPA, John Anderson Bldg,107 Rottenrow, Glasgow G4 0NG, Lanark, Scotland
2.Univ Strathclyde, Strathclyde Inst Pharm & Biomed Sci, Glasgow G4 0RE, Lanark, Scotland
3.Xian Inst Opt & Precis Mech, Xian 710049, Shanxi, Peoples R China
推荐引用方式
GB/T 7714
Sapermsap, Natakorn,Li, David Day-Uei,Al-Hemedawi, Ryath,et al. A rapid analysis platform for investigating the cellular locations of bacteria using two-photon fluorescence lifetime imaging microscopy[J]. METHODS AND APPLICATIONS IN FLUORESCENCE,2020,8(3).
APA Sapermsap, Natakorn.,Li, David Day-Uei.,Al-Hemedawi, Ryath.,Li, Yahui.,Yu, Jun.,...&Chen, Yu.(2020).A rapid analysis platform for investigating the cellular locations of bacteria using two-photon fluorescence lifetime imaging microscopy.METHODS AND APPLICATIONS IN FLUORESCENCE,8(3).
MLA Sapermsap, Natakorn,et al."A rapid analysis platform for investigating the cellular locations of bacteria using two-photon fluorescence lifetime imaging microscopy".METHODS AND APPLICATIONS IN FLUORESCENCE 8.3(2020).
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
A rapid analysis pla(2817KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Sapermsap, Natakorn]的文章
[Li, David Day-Uei]的文章
[Al-Hemedawi, Ryath]的文章
百度学术
百度学术中相似的文章
[Sapermsap, Natakorn]的文章
[Li, David Day-Uei]的文章
[Al-Hemedawi, Ryath]的文章
必应学术
必应学术中相似的文章
[Sapermsap, Natakorn]的文章
[Li, David Day-Uei]的文章
[Al-Hemedawi, Ryath]的文章
相关权益政策
暂无数据
收藏/分享
文件名: A rapid analysis platform for investigating the cellular locations of bacteria using two-photon fluorescence lifetime imaging microscopy.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。