Axial plane single-molecule super-resolution microscopy of whole cells | |
An, Sha1,2,3,4; Ziegler, Karl Ferdinand1; Zhang, Peiyi1; Wang, Yu5,6; Kwok, Tim7; Xu, Fan1; Bi, Cheng1; Matosevic, Sandro8; Yin, Peng5,6; Li, Tongcang2,9; Huang, Fang1,10,11 | |
作者部门 | 瞬态光学研究室 |
2020 | |
发表期刊 | BIOMEDICAL OPTICS EXPRESS |
ISSN | 2156-7085 |
卷号 | 11期号:1页码:461-479 |
产权排序 | 1 |
摘要 | Fluorescence nanoscopy has become an indispensable tool for studying organelle structures, protein dynamics, and interactions in biological sciences. Single-molecule localization microscopy can now routinely achieve 10-50 nm resolution through fluorescently labeled specimens in lateral optical sections. However, visualizing structures organized along the axial direction demands scanning and imaging each of the lateral imaging planes with fine intervals throughout the whole cell. This iterative process suffers from photobleaching of tagged probes, is susceptible to alignment artifacts and also limits the imaging speed. Here, we focused on the axial plane super-resolution imaging which integrated the single-objective light-sheet illumination and axial plane optical imaging with single-molecule localization technique to resolve nanoscale cellular architectures along the axial (or depth) dimension without scanning. We demonstrated that this method is compatible with DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) and exchange-PAINT by virtue of its light-sheet illumination, allowing multiplexed super-resolution imaging throughout the depth of whole cells. We further demonstrated this proposed system by resolving the axial distributions of intracellular organelles such as microtubules, mitochondria, and nuclear pore complexes in both COS-7 cells and glioblastoma patient-derived tumor cells. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement |
DOI | 10.1364/BOE.377890 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000519061700034 |
出版者 | OPTICAL SOC AMER |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.opt.ac.cn/handle/181661/93343 |
专题 | 瞬态光学研究室 |
作者单位 | 1.Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47907 USA 2.Purdue Univ, Dept Phys & Astron, W Lafayette, IN 47907 USA 3.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian 710119, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA 6.Harvard Med Sch, Dept Syst Biol, Boston, MA 02115 USA 7.Purdue Univ, Birck Nanotechnol Ctr, W Lafayette, IN 47907 USA 8.Purdue Univ, Dept Ind & Phys Pharm, W Lafayette, IN 47907 USA 9.Purdue Univ, Sch Elect & Comp Engn, W Lafayette, IN 47907 USA 10.Purdue Univ, Purdue Inst Integrat Neurosci, W Lafayette, IN 47907 US 11.Purdue Univ, Purdue Inst Inflammat Immunol & Infect Dis, W Lafayette, IN 47907 USA |
推荐引用方式 GB/T 7714 | An, Sha,Ziegler, Karl Ferdinand,Zhang, Peiyi,et al. Axial plane single-molecule super-resolution microscopy of whole cells[J]. BIOMEDICAL OPTICS EXPRESS,2020,11(1):461-479. |
APA | An, Sha.,Ziegler, Karl Ferdinand.,Zhang, Peiyi.,Wang, Yu.,Kwok, Tim.,...&Huang, Fang.(2020).Axial plane single-molecule super-resolution microscopy of whole cells.BIOMEDICAL OPTICS EXPRESS,11(1),461-479. |
MLA | An, Sha,et al."Axial plane single-molecule super-resolution microscopy of whole cells".BIOMEDICAL OPTICS EXPRESS 11.1(2020):461-479. |
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文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
Axial plane single-m(11735KB) | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 请求全文 |
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