| Total-internal-reflection fluorescence microscopy with W-shaped axicon mirrors | |
Lei, Ming1,2,3 ; Zumbusch, Andreas1,2; Lei Ming
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| 作者部门 | 瞬态光学国家重点实验室 |
| 2010-12-01 | |
| 发表期刊 | OPTICS LETTERS
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| ISSN | 0146-9592 |
| 卷号 | 35期号:23页码:4057-4059 |
| 摘要 | A scheme based on a W-shaped axicon mirror device for total-internal-reflection fluorescence microscopy (TIRFM) is presented. This approach combines the advantages of higher efficiency compared with traditional TIRFM, adjustable illumination area, and simple switching between wide-field and TIRF imaging modes. TIRF images obtained with this approach are free of shadow artifacts and of interference fringes. Example micrographs of fluorescently labeled polystyrene beads, of Convallaria majalis tissue, and of Propidium-iodide-labeled Chinese hamster ovary cells are shown, and the capabilities of the scheme are discussed. (C) 2010 Optical Society of America |
| 文章类型 | Article |
| 学科领域 | 显微镜、望远镜 |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 收录类别 | SCI ; EI |
| 语种 | 英语 |
| WOS研究方向 | Optics |
| WOS类目 | Optics |
| WOS记录号 | WOS:000284832200067 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.opt.ac.cn/handle/181661/8357 |
| 专题 | 瞬态光学研究室 |
| 通讯作者 | Lei Ming |
| 作者单位 | 1.Univ Konstanz, Dept Chem, D-78457 Constance, Germany 2.Univ Konstanz, Ctr Appl Photon, D-78457 Constance, Germany 3.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian 710119, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lei, Ming,Zumbusch, Andreas,Lei Ming. Total-internal-reflection fluorescence microscopy with W-shaped axicon mirrors[J]. OPTICS LETTERS,2010,35(23):4057-4059. |
| APA | Lei, Ming,Zumbusch, Andreas,&Lei Ming.(2010).Total-internal-reflection fluorescence microscopy with W-shaped axicon mirrors.OPTICS LETTERS,35(23),4057-4059. |
| MLA | Lei, Ming,et al."Total-internal-reflection fluorescence microscopy with W-shaped axicon mirrors".OPTICS LETTERS 35.23(2010):4057-4059. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Total-internal-refle（215KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 请求全文 | |
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