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Super-resolution imaging reveals changes in Escherichia coli SSB localization in response to DNA damage
Zhao, Tianyu1; Liu, Yan2,3; Wang, Zilin4,5; He, Rongyan2,3; Zhang, Jia Xiang4,5; Xu, Feng2,3; Lei, Ming1; Deci, Michael B.6; Nguyen, Juliane6; Bianco, Piero R.4,5
作者部门瞬态光学研究室
发表期刊GENES TO CELLS
ISSN1356-9597;1365-2443
产权排序1
摘要

The E. coli single-stranded DNA-binding protein (SSB) is essential to viability. It plays key roles in DNA metabolism where it binds to nascent single strands of DNA and to target proteins known as the SSB interactome. There are >2,000 tetramers of SSB per cell with 100-150 associated with the genome at any one time, either at DNA replication forks or at sites of repair. The remaining 1,900 tetramers could constantly diffuse throughout the cytosol or be associated with the inner membrane as observed for other DNA metabolic enzymes. To visualize SSB localization and to ascertain potential spatiotemporal changes in response to DNA damage, SSB-GFP chimeras were visualized using a novel, super-resolution microscope optimized for the study of prokaryotic cells. In the absence of DNA damage, SSB localizes to a small number of foci and the excess protein is associated with the inner membrane where it binds to the major phospholipids. Within five minutes following DNA damage, the vast majority of SSB disengages from the membrane and is found almost exclusively in the cell interior. Here, it is observed in a large number of foci, in discreet structures or, in diffuse form spread over the genome, thereby enabling repair events.

关键词DNA repair DNA replication E coli OB-fold single-strand DNA-binding protein SSB interactome super-resolution microscopy
DOI10.1111/gtc.12729
收录类别SCI
语种英语
WOS记录号WOS:000496241900001
出版者WILEY
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被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.opt.ac.cn/handle/181661/31924
专题瞬态光学研究室
通讯作者Lei, Ming
作者单位1.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian 710119, Shaanxi, Peoples R China
2.Xi An Jiao Tong Univ, Sch Life Sci & Technol, Key Lab Biomed Informat Engn, Minist Educ, Xian, Shaanxi, Peoples R China
3.Xi An Jiao Tong Univ, BEBC, Xian, Shaanxi, Peoples R China
4.Univ Buffalo, Ctr Single Mol Biophys, Buffalo, NY 14214 USA
5.Univ Buffalo, Dept Microbiol & Immunol, Buffalo, NY 14214 USA
6.Univ Buffalo, Sch Pharm, Dept Pharmaceut Sci, Buffalo, NY 14214 USA
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GB/T 7714
Zhao, Tianyu,Liu, Yan,Wang, Zilin,et al. Super-resolution imaging reveals changes in Escherichia coli SSB localization in response to DNA damage[J]. GENES TO CELLS.
APA Zhao, Tianyu.,Liu, Yan.,Wang, Zilin.,He, Rongyan.,Zhang, Jia Xiang.,...&Bianco, Piero R..
MLA Zhao, Tianyu,et al."Super-resolution imaging reveals changes in Escherichia coli SSB localization in response to DNA damage".GENES TO CELLS
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