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Quantitative phase imaging of cells in a flow cytometry arrangement utilizing Michelson interferometer-based off-axis digital holographic microscopy
Min, Junwei1,2; Yao, Baoli2; Trendafilova, Veselina1; Ketelhut, Steffi1; Kastl, Lena1; Greve, Burkhard3; Kemper, Bjoern1
作者部门瞬态光学研究室
2019-09
发表期刊JOURNAL OF BIOPHOTONICS
ISSN1864-063X;1864-0648
卷号12期号:9
产权排序1
摘要

We combined Michelson-interferometer-based off-axis digital holographic microscopy (DHM) with a common flow cytometry (FCM) arrangement. Utilizing object recognition procedures and holographic autofocusing during the numerical reconstruction of the acquired off-axis holograms, sharply focused quantitative phase images of suspended cells in flow were retrieved without labeling, from which biophysical cellular features of distinct cells, such as cell radius, refractive index and dry mass, can be subsequently retrieved in an automated manner. The performance of the proposed concept was first characterized by investigations on microspheres that were utilized as test standards. Then, we analyzed two types of pancreatic tumor cells with different morphology to further verify the applicability of the proposed method for quantitative live cell imaging. The retrieved biophysical datasets from cells in flow are found in good agreement with results from comparative investigations with previously developed DHM methods under static conditions, which demonstrates the effectiveness and reliability of our approach. Our results contribute to the establishment of DHM in imaging FCM and prospect to broaden the application spectrum of FCM by providing complementary quantitative imaging as well as additional biophysical cell parameters which are not accessible in current high-throughput FCM measurements.

关键词autofocusing digital holographic microscopy flow cytometry image flow cytometry label-free cytometry quantitative microscopy quantitative phase imaging
DOI10.1002/jbio.201900085
收录类别SCI
语种英语
WOS记录号WOS:000486778200017
出版者WILEY-V C H VERLAG GMBH
引用统计
被引频次:34[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.opt.ac.cn/handle/181661/31882
专题瞬态光学研究室
通讯作者Kemper, Bjoern
作者单位1.Univ Munster, Biomed Technol Ctr, Mendelstr 17, D-48149 Munster, Germany
2.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian, Shaanxi, Peoples R China
3.Univ Hosp Muenster, Dept Radiotherapy Radiooncol, Munster, Germany
推荐引用方式
GB/T 7714
Min, Junwei,Yao, Baoli,Trendafilova, Veselina,et al. Quantitative phase imaging of cells in a flow cytometry arrangement utilizing Michelson interferometer-based off-axis digital holographic microscopy[J]. JOURNAL OF BIOPHOTONICS,2019,12(9).
APA Min, Junwei.,Yao, Baoli.,Trendafilova, Veselina.,Ketelhut, Steffi.,Kastl, Lena.,...&Kemper, Bjoern.(2019).Quantitative phase imaging of cells in a flow cytometry arrangement utilizing Michelson interferometer-based off-axis digital holographic microscopy.JOURNAL OF BIOPHOTONICS,12(9).
MLA Min, Junwei,et al."Quantitative phase imaging of cells in a flow cytometry arrangement utilizing Michelson interferometer-based off-axis digital holographic microscopy".JOURNAL OF BIOPHOTONICS 12.9(2019).
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