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Multi-view object topography measurement with optical sectioning structured illumination microscopy
Ren, Feifei1,2; Wang, Zhaojun1,2; Qian, Jia1,2; Liang, Yansheng1; Dang, Shipei1,2; Cai, Yanan1,2; Bianco, Piero R.3; Yao, Baoli1; Lei, Ming1
Department瞬态光学技术国家重点实验室
2019-08-10
Source PublicationApplied Optics
ISSN1559128X;21553165
Volume58Issue:23Pages:6288-6294
Contribution Rank1
Abstract

Various optical instruments have been developed for three-dimensional (3D) surface topography, including the white light interference, reflectance confocal microscopes, and digital holographic microscopes, etc. However, the steep local slope of objects may cause the light to be reflected in a way that it will not be captured by the objective lens because of the finite collection angle of the objective. To solve this "shadow problem," we report a method to enlarge the collection angle range of optical sectioning structured illumination microscopy by capturing sectioned images of the objects from multiple angle of views. We develop a multi-view image fusion algorithm to reconstruct a single 3D image. Using this method, we detect previously invisible details whose slopes are beyond the collection angle of the objective. The proposed approach is useful for height map measurement and quantitative analyses in a variety of fields, such as biology, materials science, microelectronics, etc. © 2019 Optical Society of America.

DOI10.1364/AO.58.006288
Indexed BySCI ; EI
Language英语
WOS IDWOS:000480414100013
PublisherOSA - The Optical Society
EI Accession Number20193307307652
Citation statistics
Document Type期刊论文
Identifierhttp://ir.opt.ac.cn/handle/181661/31696
Collection瞬态光学技术国家重点实验室
Corresponding AuthorLei, Ming
Affiliation1.China State Key Laboratory of Transient Optics and Photonics, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an; 710119, China;
2.University of Chinese Academy of Sciences, Beijing; 100049, China;
3.Department of Microbiology and Immunology, Department of Biochemistry, Center for Single Molecule Biophysics, 321 Carry Hall, University at Buffalo, Buffalo; NY; 14214, United States
Recommended Citation
GB/T 7714
Ren, Feifei,Wang, Zhaojun,Qian, Jia,et al. Multi-view object topography measurement with optical sectioning structured illumination microscopy[J]. Applied Optics,2019,58(23):6288-6294.
APA Ren, Feifei.,Wang, Zhaojun.,Qian, Jia.,Liang, Yansheng.,Dang, Shipei.,...&Lei, Ming.(2019).Multi-view object topography measurement with optical sectioning structured illumination microscopy.Applied Optics,58(23),6288-6294.
MLA Ren, Feifei,et al."Multi-view object topography measurement with optical sectioning structured illumination microscopy".Applied Optics 58.23(2019):6288-6294.
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