On-line dynamic detection in the column chromatography separation based on an optical fiber surface plasmon resonance sensor | |
Cao, Jiaming1; Yang, Xinghua1; Teng, Pingping1; Liu, Zhihai1; Yang, Jun1; Zhang, Jianzhong1; Zhang, Yu1; Luo, Meng1; Gao, Danheng1; Kong, Depeng2; Xia, Siyuan1; Zhao, Enming1; Yuan, Libo1,3 | |
作者部门 | 信息光子学研究室 |
2019 | |
发表期刊 | Applied Optics |
ISSN | 1559128X;21553165 |
卷号 | 58期号:21页码:5774-5779 |
产权排序 | 2 |
摘要 | In this design, we introduced a surface plasmon resonance (SPR) fiber-sensing probe into a column chromatography (CC) system to realize on-line dynamic detection in sample separation. The refractive index of the gel around the probe would be adjusted dynamically by the concentration change of the sample during CC separation. To demonstrate the separation and on-line detection process, bovine serum albumin (BSA) and riboflavin-5-phosphate sodium (FMN-Na) are chosen as the analytes in a Sephadex gel filtration chromatography system. The results show that the apparent reversible shift of the SPR spectrum can characterize the separation process. Specifically, the separated BSA with an outflow time of 8 min can cause a resonance wavelength shift of 15.5 nm, and the FMN-Na with an outflow time of 26 min can cause a shift of 8.4 nm. This on-line dynamic detection of SPR spectra has great potential to save time and simplify the analysis process compared to the complex thin layer chromatography detection steps in traditional manual CC. © 2019 Optical Society of America |
DOI | 10.1364/AO.58.005774 |
收录类别 | SCI ; EI |
语种 | 英语 |
WOS记录号 | WOS:000476625600022 |
出版者 | OSA - The Optical Society |
EI入藏号 | 20192907217558 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.opt.ac.cn/handle/181661/31603 |
专题 | 信息光子学研究室 |
通讯作者 | Yang, Xinghua |
作者单位 | 1.Key Laboratory of In-Fiber Integrated Optics, Ministry of Education, College of Science, Harbin Engineering University, Harbin; 150001, China; 2.State Key Laboratory of Transient Optics and Photonics, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an; 710119, China; 3.Photonics Research Center, Guilin University of Electronics Technology, Guilin; 541004, China |
推荐引用方式 GB/T 7714 | Cao, Jiaming,Yang, Xinghua,Teng, Pingping,et al. On-line dynamic detection in the column chromatography separation based on an optical fiber surface plasmon resonance sensor[J]. Applied Optics,2019,58(21):5774-5779. |
APA | Cao, Jiaming.,Yang, Xinghua.,Teng, Pingping.,Liu, Zhihai.,Yang, Jun.,...&Yuan, Libo.(2019).On-line dynamic detection in the column chromatography separation based on an optical fiber surface plasmon resonance sensor.Applied Optics,58(21),5774-5779. |
MLA | Cao, Jiaming,et al."On-line dynamic detection in the column chromatography separation based on an optical fiber surface plasmon resonance sensor".Applied Optics 58.21(2019):5774-5779. |
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On-line dynamic dete(1690KB) | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 请求全文 |
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