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Label-free detecting oligonucleotide hybridization melting temperature in real-time with a reflectometric interference spectroscopy–based nanosensor system
Lu, Yao1; Sun, Dan1; Wang, Kaige1; Bai, Xiaohong2; Zhang, Chen1; Zhao, Wei1; Feng, Xiaoqiang1; Bai, Jintao1
Department精密物理量测量实验室
2019-09
Source PublicationOptik
ISSN00304026
Volume192
Contribution Rank2
Abstract

The accurate determination of melting temperature (Tm) of oligonucleotide hybridization is the primary for various biosensors based on the technique of using surface-tethered nucleic acids as probes. In this paper, a new type of nanosensor based on the reflectometric interference spectroscopy (RIFS) was proposed to determine the Tm in real time. A composite solid nanostructure, i.e., a nanoporous anodic alumina film with a 15 nm top layer of gold sputtered on surface, was employed as the substrate of the RIFS-based nanosensor, and a 30 bp oligonucleotide molecule was bond to the inner wall of pores and hybridized, and then its Tm was detected with raising temperature. The experimental data showed that there were obvious signs of dsDNA molecules denatured and melted into ssDNA molecules at 95 , which was highly consistent with the theoretical analysis. The cost-effective RIFS nanosensor has excellent thermal and physicochemical stabilities, higher determination accuracy, and great potential in the field of biomolecule research. © 2019 Elsevier GmbH

KeywordReflectometric interference spectroscopy Nanosensor Oligonucleotide hybridization Melting temperature Label free sensing
DOI10.1016/j.ijleo.2019.06.003
Indexed BySCI ; EI
Language英语
WOS IDWOS:000494471400006
PublisherElsevier GmbH
EI Accession Number20192507071490
Citation statistics
Document Type期刊论文
Identifierhttp://ir.opt.ac.cn/handle/181661/31554
Collection精密物理量测量实验室
Corresponding AuthorWang, Kaige
Affiliation1.Institute of Photonics & Photon-Technology, National Center for International Research of Photoelectric Technology & Nano-functional Materials,Northwest University, Xi'an; 710069, China;
2.Xi'An Institute of Optics & Precision Mechanics, CAS, Xi'an; 710119, China
Recommended Citation
GB/T 7714
Lu, Yao,Sun, Dan,Wang, Kaige,等. Label-free detecting oligonucleotide hybridization melting temperature in real-time with a reflectometric interference spectroscopy–based nanosensor system[J]. Optik,2019,192.
APA Lu, Yao.,Sun, Dan.,Wang, Kaige.,Bai, Xiaohong.,Zhang, Chen.,...&Bai, Jintao.(2019).Label-free detecting oligonucleotide hybridization melting temperature in real-time with a reflectometric interference spectroscopy–based nanosensor system.Optik,192.
MLA Lu, Yao,et al."Label-free detecting oligonucleotide hybridization melting temperature in real-time with a reflectometric interference spectroscopy–based nanosensor system".Optik 192(2019).
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