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Simple and fast spectral domain algorithm for quantitative phase imaging of living cells with digital holographic microscopy
Min, Junwei1,2; Yao, Baoli2; Ketelhut, Steffi1; Kemper, Bjoern1; Kemper, B (reprint author), Univ Munster, Biomed Technol Ctr, Mendelstr 17, D-48149 Munster, Germany.
2017
会议名称Conference on Three-Dimensional and Multidimensional Microscopy - Image Acquisition and Processing XXIV
会议录名称THREE-DIMENSIONAL AND MULTIDIMENSIONAL MICROSCOPY: IMAGE ACQUISITION AND PROCESSING XXIV
卷号10070
会议日期2017-01-30
会议地点San Francisco, CA
出版者SPIE-INT SOC OPTICAL ENGINEERING
产权排序1
摘要

The modular combination of optical microscopes with digital holographic microscopy (DHM) has been proven to be a powerful tool for quantitative live cell imaging. The introduction of condenser and different microscope objectives (MO) simplifies the usage of the technique and makes it easier to measure different kinds of specimens with different magnifications. However, the high flexibility of illumination and imaging also causes variable phase aberrations that need to be eliminated for high resolution quantitative phase imaging. The existent phase aberrations compensation methods either require add additional elements into the reference arm or need specimen free reference areas or separate reference holograms to build up suitable digital phase masks. These inherent requirements make them unpractical for usage with highly variable illumination and imaging systems and prevent on-line monitoring of living cells. In this paper, we present a simple numerical method for phase aberration compensation based on the analysis of holograms in spatial frequency domain with capabilities for on-line quantitative phase imaging. From a single shot off-axis hologram, the whole phase aberration can be eliminated automatically without numerical fitting or pre-knowledge of the setup. The capabilities and robustness for quantitative phase imaging of living cancer cells are demonstrated.

关键词Digital Holographic Microscopy Aberration Compensation Frequency Spectrum Analysis Quantitative Phase Imaging Quantitative Live Cell Analysis
学科领域Microscopy
作者部门瞬态光学技术国家重点实验室
DOI10.1117/12.2252883
收录类别EI ; ISTP
ISBN号978-1-5106-0581-7; 978-1-5106-0582-4
语种英语
ISSN号0277-786X
引用统计
文献类型会议论文
条目标识符http://ir.opt.ac.cn/handle/181661/29195
专题瞬态光学研究室
通讯作者Kemper, B (reprint author), Univ Munster, Biomed Technol Ctr, Mendelstr 17, D-48149 Munster, Germany.
作者单位1.Univ Munster, Biomed Technol Ctr, Mendelstr 17, D-48149 Munster, Germany
2.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian 710119, Shaanxi, Peoples R China
推荐引用方式
GB/T 7714
Min, Junwei,Yao, Baoli,Ketelhut, Steffi,et al. Simple and fast spectral domain algorithm for quantitative phase imaging of living cells with digital holographic microscopy[C]:SPIE-INT SOC OPTICAL ENGINEERING,2017.
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