Mechanisms of pulse response and differential response of bacteriorhodopsin and their relations

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	Mechanisms of pulse response and differential response of bacteriorhodopsin and their relations
	Yao, BL ; Wang, YL ; Hu, KS ; Chen, DL ; Zheng, Y ; Lei, M
	2002-11-01
发表期刊	PHOTOCHEMISTRY AND PHOTOBIOLOGY
卷号	76 期号:5 页码:545-548
摘要	Bacteriorhodopsin (BR) films are oriented and deposited on indium tin oxide conductive glass by using electrophoretic sedimentation and Langmuir-Blodgett methods to construct sandwich-type photocells, respectively. The pulse response photoelectric signal of the BR photocell under pulsed laser and the differential response photoelectric signal under irradiation of interval light are measured. The origins of these two types of photoelectric responses and their correlations are analyzed. The pulse response signal initiates from the ultrafast charge separation of the retinal and the proton translocation followed by the deprotonation and reprotonation of the Schiff base and its surrounding amino acids. This is a quick response and is the preceding reaction of the differential response. The differential response signal is caused by the charging and discharging of the continuous proton current of the BR light-driven proton pump at light-on and light-off, which is a slow process. The differential response is related to not only the construction of the BR photocell but also the coupling mode of measurement. To observe the differential response signal, the BR photocell must have large enough B-3 and B-3' components in its pulse response as well as an alternative coupling mode to measure it.
文章类型	Article
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
收录类别	SCI
关键词[WOS]	LIGHT ; SIGNALS
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
WOS记录号	WOS:000179368700013
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.opt.ac.cn/handle/181661/25445
专题	瞬态光学研究室
作者单位	1.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt Technol, Xian, Peoples R China 2.Chinese Acad Sci, Inst Biophys, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Yao, BL,Wang, YL,Hu, KS,et al. Mechanisms of pulse response and differential response of bacteriorhodopsin and their relations[J]. PHOTOCHEMISTRY AND PHOTOBIOLOGY,2002,76(5):545-548.
APA	Yao, BL,Wang, YL,Hu, KS,Chen, DL,Zheng, Y,&Lei, M.(2002).Mechanisms of pulse response and differential response of bacteriorhodopsin and their relations.PHOTOCHEMISTRY AND PHOTOBIOLOGY,76(5),545-548.
MLA	Yao, BL,et al."Mechanisms of pulse response and differential response of bacteriorhodopsin and their relations".PHOTOCHEMISTRY AND PHOTOBIOLOGY 76.5(2002):545-548.

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