| Time-resolved two-photon excitation fluorescence spectroscopy | |
| Liu Li-Xin; Qu Jun-Le; Lin Zi-Yang; Chen Dan-Ni; Xu Gai-Xia; Hu Tao; Guo Bao-Ping; Niu Han-Ben | |
| 2006-12-01 | |
| 发表期刊 | ACTA PHYSICA SINICA
 |
| 卷号 | 55期号:12页码:6281-6286 |
| 摘要 | A novel time-resolved two-photon excitation fluorescence spectroscopic system based on a high repetition rate streak camera and capable of simultaneous measurement of fluorescence spectrum and lifetime of the samples is presented. The system has high temporal (6.5-200 ps) and spectral ( 1-3 nm) resolutions and permits rapid data acquisition and reliable and reproducible lifetime and spectrum determinations. The system is tested with standard fluorescent dyes (rhodamine 6G, coumarin 314 and their mixtures) and the lifetimes and spectra obtained agree well with those reported in the literatures. Experimental results indicate that our system provides a unique contrast mechanism for discriminating multi-fluorophore or multi-component of biological tissues through spectrally and temporally resolved information and may find extensive applications in multispectral fluorescence lifetime imaging and fluorescence resonance energy transfer imaging. |
| 文章类型 | Article |
| 关键词 | Fluorescence Lifetime Fluorescence Spectrum Two-photon Excitation High Repetition Rate Streak Camera |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 收录类别 | SCI ; EI |
| 关键词[WOS] | IMAGING MICROSCOPY ; STREAK CAMERA ; QUANTUM DOTS ; LIFETIME ; DIAGNOSTICS ; CELLS |
| 语种 | 中文 |
| WOS研究方向 | Physics |
| WOS类目 | Physics, Multidisciplinary |
| WOS记录号 | WOS:000243900800015 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.opt.ac.cn/handle/181661/25178 |
| 专题 | 研究生部 |
| 作者单位 | 1.Shenzen Univ, Inst Optoelect, Shenzhen 518060, Peoples R China 2.Chinese Acad Sci, Xian Inst Opt & Precis Mech, Xian 710068, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu Li-Xin,Qu Jun-Le,Lin Zi-Yang,et al. Time-resolved two-photon excitation fluorescence spectroscopy[J]. ACTA PHYSICA SINICA,2006,55(12):6281-6286. |
| APA | Liu Li-Xin.,Qu Jun-Le.,Lin Zi-Yang.,Chen Dan-Ni.,Xu Gai-Xia.,...&Niu Han-Ben.(2006).Time-resolved two-photon excitation fluorescence spectroscopy.ACTA PHYSICA SINICA,55(12),6281-6286. |
| MLA | Liu Li-Xin,et al."Time-resolved two-photon excitation fluorescence spectroscopy".ACTA PHYSICA SINICA 55.12(2006):6281-6286. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| 双光子激发时间分辨荧光光谱测量技术.pd（431KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 请求全文 | |
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