| Double-Exposure Optical Sectioning Structured Illumination Microscopy Based on Hilbert Transform Reconstruction | |
Zhou, Xing1,2; Lei, Ming1 ; Dan, Dan1; Yao, Baoli1; Qian, Jia1; Yan, Shaohui1; Yang, Yanlong1; Min, Junwei1; Peng, Tong1; Ye, Tong3; Chen, Guangde2
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| 2015-03-23 | |
| 发表期刊 | PLOS ONE
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| 卷号 | 10期号:3 |
| 摘要 | Structured illumination microscopy (SIM) with axially optical sectioning capability has found widespread applications in three-dimensional live cell imaging in recent years, since it combines high sensitivity, short image acquisition time, and high spatial resolution. To obtain one sectioned slice, three raw images with a fixed phase-shift, normally 2 pi/3, are generally required. In this paper, we report a data processing algorithm based on the one-dimensional Hilbert transform, which needs only two raw images with arbitrary phase-shift for each single slice. The proposed algorithm is different from the previous two-dimensional Hilbert spiral transform algorithm in theory. The presented algorithm has the advantages of simpler data processing procedure, faster computation speed and better reconstructed image quality. The validity of the scheme is verified by imaging biological samples in our developed DMD-based LED-illumination SIM system. |
| 文章类型 | Article |
| WOS标题词 | Science & Technology |
| DOI | 10.1371/journal.pone.0120892 |
| 收录类别 | SCI |
| 关键词[WOS] | 2-DIMENSIONAL FRINGE PATTERNS ; PHASE QUADRATURE TRANSFORM ; NATURAL DEMODULATION ; PLANE ILLUMINATION ; LIGHT |
| 语种 | 英语 |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| WOS记录号 | WOS:000351987300188 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.opt.ac.cn/handle/181661/24134 |
| 专题 | 瞬态光学研究室 |
| 作者单位 | 1.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian 710119, Peoples R China 2.Xi An Jiao Tong Univ, Sch Sci, Xian 710119, Peoples R China 3.Clemson Univ, Dept Bioengn, Clemson MUSC Bioengn Program, Charleston, SC 29425 USA |
| 推荐引用方式 GB/T 7714 | Zhou, Xing,Lei, Ming,Dan, Dan,et al. Double-Exposure Optical Sectioning Structured Illumination Microscopy Based on Hilbert Transform Reconstruction[J]. PLOS ONE,2015,10(3). |
| APA | Zhou, Xing.,Lei, Ming.,Dan, Dan.,Yao, Baoli.,Qian, Jia.,...&Chen, Guangde.(2015).Double-Exposure Optical Sectioning Structured Illumination Microscopy Based on Hilbert Transform Reconstruction.PLOS ONE,10(3). |
| MLA | Zhou, Xing,et al."Double-Exposure Optical Sectioning Structured Illumination Microscopy Based on Hilbert Transform Reconstruction".PLOS ONE 10.3(2015). |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Double-Exposure Opti（1844KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY | 请求全文 | |
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