Schlieren confocal microscopy for phase-relief imaging | |
Xie, Hao1,2,3; Jin, Dayong4; Yu, Junjie5; Peng, Tong1,6; Ding, Yichen1; Zhou, Changhe5; Xi, Peng1 | |
作者部门 | 瞬态光学国家重点实验室 |
2014-03-01 | |
发表期刊 | OPTICS LETTERS |
ISSN | 0146-9592 |
卷号 | 39期号:5页码:1238-1241 |
摘要 | We demonstrate a simple phase-sensitive microscopic technique capable of imaging the phase gradient of a transparent specimen, based on the Schlieren modulation and confocal laser scanning microscopy (CLSM). The incident laser is refracted by the phase gradient of the specimen and excites a fluorescence plate behind the specimen to create a secondary illumination; then the fluoresence is modulated by a partial obstructor before entering the confocal pinhole. The quantitative relationship between the image intensity and the sample phase gradient can be derived. This setup is very easy to be adapted to current confocal setups, so that multimodality fluorescence/structure images can be obtained within a single system. (C) 2014 Optical Society of America |
文章类型 | Article |
WOS标题词 | Science & Technology ; Physical Sciences |
DOI | 10.1364/OL.39.001238 |
收录类别 | SCI ; EI |
关键词[WOS] | CONTRAST ; CELLS |
语种 | 英语 |
WOS研究方向 | Optics |
WOS类目 | Optics |
WOS记录号 | WOS:000332233300035 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.opt.ac.cn/handle/181661/22207 |
专题 | 研究生部 |
作者单位 | 1.Peking Univ, Dept Biomed Engn, Coll Engn, Beijing 100871, Peoples R China 2.Georgia Inst Technol, Wallace H Coulter Dept Biomed Engn, Atlanta, GA 30332 USA 3.Emory Univ, Atlanta, GA 30332 USA 4.Macquarie Univ, MQ Photon Res Ctr, Adv Cytometry Labs, Sydney, NSW 2109, Australia 5.Chinese Acad Sci, Shanghai Inst Opt & Fine Mech, Shanghai 201800, Peoples R China 6.Chinese Acad Sci, Xian Inst Opt & Precis Mech, Xian 710068, Peoples R China |
推荐引用方式 GB/T 7714 | Xie, Hao,Jin, Dayong,Yu, Junjie,et al. Schlieren confocal microscopy for phase-relief imaging[J]. OPTICS LETTERS,2014,39(5):1238-1241. |
APA | Xie, Hao.,Jin, Dayong.,Yu, Junjie.,Peng, Tong.,Ding, Yichen.,...&Xi, Peng.(2014).Schlieren confocal microscopy for phase-relief imaging.OPTICS LETTERS,39(5),1238-1241. |
MLA | Xie, Hao,et al."Schlieren confocal microscopy for phase-relief imaging".OPTICS LETTERS 39.5(2014):1238-1241. |
条目包含的文件 | ||||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
Schlieren confocal m(345KB) | 期刊论文 | 出版稿 | 限制开放 | CC BY | 请求全文 |
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