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Schlieren confocal microscopy for phase-relief imaging
Xie, Hao1,2,3; Jin, Dayong4; Yu, Junjie5; Peng, Tong1,6; Ding, Yichen1; Zhou, Changhe5; Xi, Peng1
作者部门瞬态光学国家重点实验室
2014-03-01
发表期刊OPTICS LETTERS
ISSN0146-9592
卷号39期号:5页码:1238-1241
摘要We demonstrate a simple phase-sensitive microscopic technique capable of imaging the phase gradient of a transparent specimen, based on the Schlieren modulation and confocal laser scanning microscopy (CLSM). The incident laser is refracted by the phase gradient of the specimen and excites a fluorescence plate behind the specimen to create a secondary illumination; then the fluoresence is modulated by a partial obstructor before entering the confocal pinhole. The quantitative relationship between the image intensity and the sample phase gradient can be derived. This setup is very easy to be adapted to current confocal setups, so that multimodality fluorescence/structure images can be obtained within a single system. (C) 2014 Optical Society of America
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
DOI10.1364/OL.39.001238
收录类别SCI ; EI
关键词[WOS]CONTRAST ; CELLS
语种英语
WOS研究方向Optics
WOS类目Optics
WOS记录号WOS:000332233300035
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.opt.ac.cn/handle/181661/22207
专题研究生部
作者单位1.Peking Univ, Dept Biomed Engn, Coll Engn, Beijing 100871, Peoples R China
2.Georgia Inst Technol, Wallace H Coulter Dept Biomed Engn, Atlanta, GA 30332 USA
3.Emory Univ, Atlanta, GA 30332 USA
4.Macquarie Univ, MQ Photon Res Ctr, Adv Cytometry Labs, Sydney, NSW 2109, Australia
5.Chinese Acad Sci, Shanghai Inst Opt & Fine Mech, Shanghai 201800, Peoples R China
6.Chinese Acad Sci, Xian Inst Opt & Precis Mech, Xian 710068, Peoples R China
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GB/T 7714
Xie, Hao,Jin, Dayong,Yu, Junjie,et al. Schlieren confocal microscopy for phase-relief imaging[J]. OPTICS LETTERS,2014,39(5):1238-1241.
APA Xie, Hao.,Jin, Dayong.,Yu, Junjie.,Peng, Tong.,Ding, Yichen.,...&Xi, Peng.(2014).Schlieren confocal microscopy for phase-relief imaging.OPTICS LETTERS,39(5),1238-1241.
MLA Xie, Hao,et al."Schlieren confocal microscopy for phase-relief imaging".OPTICS LETTERS 39.5(2014):1238-1241.
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