| DMD-based LED-illumination Super-resolution and optical sectioning microscopy | |
Dan, Dan1 ; Lei, Ming1; Yao, Baoli1; Wang, Wen2,3; Winterhalder, Martin4; Zumbusch, Andreas4; Qi, Yujiao1; Xia, Liang2,3; Yan, Shaohui1; Yang, Yanlong1; Gao, Peng1; Ye, Tong1; Zhao, Wei1; Lei M(雷铭)
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| 作者部门 | 瞬态光学与光子技术国家重点实验室 |
| 2013-01-23 | |
| 发表期刊 | SCIENTIFIC REPORTS
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| ISSN | 2045-2322 |
| 卷号 | 3期号:0页码:1116 |
| 产权排序 | 1 |
| 摘要 | Super-resolution three-dimensional (3D) optical microscopy has incomparable advantages over other high-resolution microscopic technologies, such as electron microscopy and atomic force microscopy, in the study of biological molecules, pathways and events in live cells and tissues. We present a novel approach of structured illumination microscopy (SIM) by using a digital micromirror device (DMD) for fringe projection and a low-coherence LED light for illumination. The lateral resolution of 90 nm and the optical sectioning depth of 120 mm were achieved. The maximum acquisition speed for 3D imaging in the optical sectioning mode was 1.63107 pixels/second, which was mainly limited by the sensitivity and speed of the CCD camera. In contrast to other SIM techniques, the DMD-based LED-illumination SIM is cost-effective, ease of multi-wavelength switchable and speckle-noise-free. The 2D super-resolution and 3D optical sectioning modalities can be easily switched and applied to either fluorescent or non-fluorescent specimens. |
| 文章类型 | Article |
| WOS标题词 | Science & Technology |
| DOI | 10.1038/srep01116 |
| 收录类别 | SCI |
| 关键词[WOS] | DIGITAL MICROMIRROR DEVICE ; SPATIAL LIGHT-MODULATOR ; STRUCTURED-ILLUMINATION ; FLUORESCENCE MICROSCOPY ; RECONSTRUCTION MICROSCOPY ; RESOLUTION LIMIT ; NANOSCOPY ; CELLS |
| 语种 | 英语 |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| WOS记录号 | WOS:000314091000009 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.opt.ac.cn/handle/181661/20945 |
| 专题 | 瞬态光学研究室 |
| 通讯作者 | Lei M(雷铭) |
| 作者单位 | 1.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian 710119, Peoples R China 2.Fourth Mil Med Univ, Dept Anat, Xian 710032, Peoples R China 3.Fourth Mil Med Univ, KK Leung Brain Res Ctr, Xian 710032, Peoples R China 4.Univ Konstanz, Dept Chem, D-78457 Constance, Germany |
| 推荐引用方式 GB/T 7714 | Dan, Dan,Lei, Ming,Yao, Baoli,et al. DMD-based LED-illumination Super-resolution and optical sectioning microscopy[J]. SCIENTIFIC REPORTS,2013,3(0):1116. |
| APA | Dan, Dan.,Lei, Ming.,Yao, Baoli.,Wang, Wen.,Winterhalder, Martin.,...&雷铭.(2013).DMD-based LED-illumination Super-resolution and optical sectioning microscopy.SCIENTIFIC REPORTS,3(0),1116. |
| MLA | Dan, Dan,et al."DMD-based LED-illumination Super-resolution and optical sectioning microscopy".SCIENTIFIC REPORTS 3.0(2013):1116. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| DMD-based LED-illumi（846KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY | 请求全文 | |
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