Compact multi-band fluorescent microscope with an electrically tunable lens for autofocusing | |
Wang, Zhaojun1; Lei, Ming1; Yao, Baoli1; Cai, Yanan1; Liang, Yansheng1; Yang, Yanlong1; Yang, Xibin2; Li, Hui2; Xiong, Daxi2 | |
作者部门 | 瞬态光学技术国家重点实验室 |
2015-11-01 | |
发表期刊 | BIOMEDICAL OPTICS EXPRESS |
ISSN | 2156-7085 |
卷号 | 6期号:11页码:4353-4364 |
产权排序 | 1 |
摘要 | Autofocusing is a routine technique in redressing focus drift that occurs in time-lapse microscopic image acquisition. To date, most automatic microscopes are designed on the distance detection scheme to fulfill the autofocusing operation, which may suffer from the low contrast of the reflected signal due to the refractive index mismatch at the water/glass interface. To achieve high autofocusing speed with minimal motion artifacts, we developed a compact multi-band fluorescent microscope with an electrically tunable lens (ETL) device for autofocusing. A modified searching algorithm based on equidistant scanning and curve fitting is proposed, which no longer requires a single-peak focus curve and then efficiently restrains the impact of external disturbance. This technique enables us to achieve an autofocusing time of down to 170 ms and the reproductivity of over 97%. The imaging head of the microscope has dimensions of 12 cm x 12 cm x 6 cm. This portable instrument can easily fit inside standard incubators for real-time imaging of living specimens. (C) 2015 Optical Society of America |
文章类型 | Article |
学科领域 | Biochemical Research Methods |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
DOI | 10.1364/BOE.6.004353 |
收录类别 | SCI ; EI |
关键词[WOS] | LIQUID-CRYSTAL ; FOCUS |
语种 | 英语 |
WOS研究方向 | Biochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging |
项目资助者 | Natural Science Foundation of China (NSFC)(61522511 ; National Basic Research Program (973 Program) of China(2012CB921900) ; 61275193 ; 81427802 ; 11404389) |
WOS类目 | Biochemical Research Methods ; Optics ; Radiology, Nuclear Medicine & Medical Imaging |
WOS记录号 | WOS:000366122400015 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.opt.ac.cn/handle/181661/27661 |
专题 | 瞬态光学研究室 |
作者单位 | 1.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian 710119, Peoples R China 2.Chinese Acad Sci, Suzhou Inst Biomed Engn & Technol, Jiangsu Key Lab Med Opt, Suzhou 215163, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Zhaojun,Lei, Ming,Yao, Baoli,et al. Compact multi-band fluorescent microscope with an electrically tunable lens for autofocusing[J]. BIOMEDICAL OPTICS EXPRESS,2015,6(11):4353-4364. |
APA | Wang, Zhaojun.,Lei, Ming.,Yao, Baoli.,Cai, Yanan.,Liang, Yansheng.,...&Xiong, Daxi.(2015).Compact multi-band fluorescent microscope with an electrically tunable lens for autofocusing.BIOMEDICAL OPTICS EXPRESS,6(11),4353-4364. |
MLA | Wang, Zhaojun,et al."Compact multi-band fluorescent microscope with an electrically tunable lens for autofocusing".BIOMEDICAL OPTICS EXPRESS 6.11(2015):4353-4364. |
条目包含的文件 | ||||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
Compact multi-band f(2090KB) | 期刊论文 | 作者接受稿 | 限制开放 | CC BY-NC-SA | 请求全文 |
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