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Compact multi-band fluorescent microscope with an electrically tunable lens for autofocusing
Wang, Zhaojun1; Lei, Ming1; Yao, Baoli1; Cai, Yanan1; Liang, Yansheng1; Yang, Yanlong1; Yang, Xibin2; Li, Hui2; Xiong, Daxi2
作者部门瞬态光学技术国家重点实验室
2015-11-01
发表期刊BIOMEDICAL OPTICS EXPRESS
ISSN2156-7085
卷号6期号:11页码:4353-4364
产权排序1
摘要Autofocusing is a routine technique in redressing focus drift that occurs in time-lapse microscopic image acquisition. To date, most automatic microscopes are designed on the distance detection scheme to fulfill the autofocusing operation, which may suffer from the low contrast of the reflected signal due to the refractive index mismatch at the water/glass interface. To achieve high autofocusing speed with minimal motion artifacts, we developed a compact multi-band fluorescent microscope with an electrically tunable lens (ETL) device for autofocusing. A modified searching algorithm based on equidistant scanning and curve fitting is proposed, which no longer requires a single-peak focus curve and then efficiently restrains the impact of external disturbance. This technique enables us to achieve an autofocusing time of down to 170 ms and the reproductivity of over 97%. The imaging head of the microscope has dimensions of 12 cm x 12 cm x 6 cm. This portable instrument can easily fit inside standard incubators for real-time imaging of living specimens. (C) 2015 Optical Society of America
文章类型Article
学科领域Biochemical Research Methods
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1364/BOE.6.004353
收录类别SCI ; EI
关键词[WOS]LIQUID-CRYSTAL ; FOCUS
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
项目资助者Natural Science Foundation of China (NSFC)(61522511 ; National Basic Research Program (973 Program) of China(2012CB921900) ; 61275193 ; 81427802 ; 11404389)
WOS类目Biochemical Research Methods ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
WOS记录号WOS:000366122400015
引用统计
被引频次:30[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.opt.ac.cn/handle/181661/27661
专题瞬态光学研究室
作者单位1.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian 710119, Peoples R China
2.Chinese Acad Sci, Suzhou Inst Biomed Engn & Technol, Jiangsu Key Lab Med Opt, Suzhou 215163, Peoples R China
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Wang, Zhaojun,Lei, Ming,Yao, Baoli,et al. Compact multi-band fluorescent microscope with an electrically tunable lens for autofocusing[J]. BIOMEDICAL OPTICS EXPRESS,2015,6(11):4353-4364.
APA Wang, Zhaojun.,Lei, Ming.,Yao, Baoli.,Cai, Yanan.,Liang, Yansheng.,...&Xiong, Daxi.(2015).Compact multi-band fluorescent microscope with an electrically tunable lens for autofocusing.BIOMEDICAL OPTICS EXPRESS,6(11),4353-4364.
MLA Wang, Zhaojun,et al."Compact multi-band fluorescent microscope with an electrically tunable lens for autofocusing".BIOMEDICAL OPTICS EXPRESS 6.11(2015):4353-4364.
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