Fast frame scanning camera system for light-sheet microscopy | |
Wu, Di; Zhou, Xing; Yao, Baoli; Li, Runze; Yang, Yanlong; Peng, Tong; Lei, Ming; Dan, Dan; Ye, Tong | |
作者部门 | 瞬态光学技术国家重点实验室 |
2015-10-10 | |
发表期刊 | APPLIED OPTICS |
ISSN | 1559128X |
卷号 | 54期号:29页码:8632-8636 |
产权排序 | 1 |
摘要 | In the interest of improving the temporal resolution for light-sheet microscopy, we designed a fast frame scanning camera system that incorporated a galvanometer scanning mirror into the imaging path of a home-built light-sheet microscope. This system transformed a temporal image sequence to a spatial one so that multiple images could be acquired during one exposure period. The improvement factor of the frame rate was dependent on the number of sub-images that could be tiled on the sensor without overlapping each other and was therefore a trade-off with the image size. As a demonstration, we achieved 960 frames/s (fps) on a CCD camera that was originally capable of recording images at only 30 fps (full frame). This allowed us to observe millisecond or sub-millisecond events with ordinary CCD cameras. (c) 2015 Optical Society of America |
文章类型 | Article |
WOS标题词 | Science & Technology ; Physical Sciences |
DOI | 10.1364/AO.54.008632 |
收录类别 | SCI ; EI |
关键词[WOS] | OPTICAL SECTIONING MICROSCOPY ; ILLUMINATION MICROSCOPY ; FLUORESCENCE MICROSCOPY ; STRUCTURED-ILLUMINATION ; PLANE ILLUMINATION ; BRAIN |
语种 | 英语 |
WOS研究方向 | Optics |
WOS类目 | Optics |
WOS记录号 | WOS:000362667200008 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.opt.ac.cn/handle/181661/25487 |
专题 | 瞬态光学研究室 |
作者单位 | Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian 710119, Peoples R China |
推荐引用方式 GB/T 7714 | Wu, Di,Zhou, Xing,Yao, Baoli,et al. Fast frame scanning camera system for light-sheet microscopy[J]. APPLIED OPTICS,2015,54(29):8632-8636. |
APA | Wu, Di.,Zhou, Xing.,Yao, Baoli.,Li, Runze.,Yang, Yanlong.,...&Ye, Tong.(2015).Fast frame scanning camera system for light-sheet microscopy.APPLIED OPTICS,54(29),8632-8636. |
MLA | Wu, Di,et al."Fast frame scanning camera system for light-sheet microscopy".APPLIED OPTICS 54.29(2015):8632-8636. |
条目包含的文件 | ||||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
Fast frame scanning (591KB) | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 请求全文 |
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