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Femtosecond laser-assisted microinjection into living neurons
Lei, Ming1; Xu, Hanpeng2; Yang, Hao2; Yao, Baoli1
2008-09-30
Source PublicationJOURNAL OF NEUROSCIENCE METHODS
Volume174Issue:2Pages:215-218
AbstractA Ti:sapphire femtosecond laser micro-manipulation system was used to perforate vital cells. Propidium iodide was successfully transferred into both astrocytes and PC12 cells via the channel of a femtosecond laser, which induced transiently recoverable opening of the cell membrane. The transfection efficiency reached almost 100%. This method also had the advantage of contact-free, non-disruptive and stable transfection. (C) 2008 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordMicroinjection Transfection Femtosecond Laser Neuron
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.jneumeth.2008.07.006
Indexed BySCI
WOS KeywordCELLS ; TRANSFECTION ; DNA
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Neurosciences & Neurology
WOS SubjectBiochemical Research Methods ; Neurosciences
WOS IDWOS:000259905600007
Citation statistics
Document Type期刊论文
Identifierhttp://ir.opt.ac.cn/handle/181661/25212
Collection瞬态光学技术国家重点实验室
Affiliation1.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian 710119, Peoples R China
2.Fourth Mil Med Univ, Inst Neurosci, Xian 710032, Peoples R China
Recommended Citation
GB/T 7714
Lei, Ming,Xu, Hanpeng,Yang, Hao,et al. Femtosecond laser-assisted microinjection into living neurons[J]. JOURNAL OF NEUROSCIENCE METHODS,2008,174(2):215-218.
APA Lei, Ming,Xu, Hanpeng,Yang, Hao,&Yao, Baoli.(2008).Femtosecond laser-assisted microinjection into living neurons.JOURNAL OF NEUROSCIENCE METHODS,174(2),215-218.
MLA Lei, Ming,et al."Femtosecond laser-assisted microinjection into living neurons".JOURNAL OF NEUROSCIENCE METHODS 174.2(2008):215-218.
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