Femtosecond laser-assisted microinjection into living neurons | |
Lei, Ming1; Xu, Hanpeng2; Yang, Hao2; Yao, Baoli1 | |
2008-09-30 | |
发表期刊 | JOURNAL OF NEUROSCIENCE METHODS |
卷号 | 174期号:2页码:215-218 |
摘要 | A Ti:sapphire femtosecond laser micro-manipulation system was used to perforate vital cells. Propidium iodide was successfully transferred into both astrocytes and PC12 cells via the channel of a femtosecond laser, which induced transiently recoverable opening of the cell membrane. The transfection efficiency reached almost 100%. This method also had the advantage of contact-free, non-disruptive and stable transfection. (C) 2008 Elsevier B.V. All rights reserved. |
文章类型 | Article |
关键词 | Microinjection Transfection Femtosecond Laser Neuron |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
DOI | 10.1016/j.jneumeth.2008.07.006 |
收录类别 | SCI |
关键词[WOS] | CELLS ; TRANSFECTION ; DNA |
语种 | 英语 |
WOS研究方向 | Biochemistry & Molecular Biology ; Neurosciences & Neurology |
WOS类目 | Biochemical Research Methods ; Neurosciences |
WOS记录号 | WOS:000259905600007 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.opt.ac.cn/handle/181661/25212 |
专题 | 瞬态光学研究室 |
作者单位 | 1.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian 710119, Peoples R China 2.Fourth Mil Med Univ, Inst Neurosci, Xian 710032, Peoples R China |
推荐引用方式 GB/T 7714 | Lei, Ming,Xu, Hanpeng,Yang, Hao,et al. Femtosecond laser-assisted microinjection into living neurons[J]. JOURNAL OF NEUROSCIENCE METHODS,2008,174(2):215-218. |
APA | Lei, Ming,Xu, Hanpeng,Yang, Hao,&Yao, Baoli.(2008).Femtosecond laser-assisted microinjection into living neurons.JOURNAL OF NEUROSCIENCE METHODS,174(2),215-218. |
MLA | Lei, Ming,et al."Femtosecond laser-assisted microinjection into living neurons".JOURNAL OF NEUROSCIENCE METHODS 174.2(2008):215-218. |
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Femtosecond laser-as(501KB) | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 请求全文 |
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