| Structured illumination microscopy for super-resolution and optical sectioning | |
Dan, Dan ; Yao, Baoli; Lei, Ming
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| 作者部门 | 瞬态光学国家重点实验室 |
| 2014-04-01 | |
| 发表期刊 | CHINESE SCIENCE BULLETIN
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| ISSN | 1001-6538 |
| 卷号 | 59期号:12页码:1291-1307 |
| 摘要 | Optical microscopy plays an essential role in biological studies due to its capability and compatibility of non-contact, minimally invasive observation and measurement of live specimens. However, the conventional optical microscopy only has a spatial resolution about 200 nm due to the Abbe diffraction limit, and also lacks the ability of three-dimensional imaging. Super-resolution far-field optical microscopy based on special illumination schemes has been dramatically developed over the last decade. Among them, only the structured illumination microscopy (SIM) is of wide-field geometry that enables it easily compatible with the conventional optical microscope. In this article, the principle of SIM was introduced in terms of point spread function (PSF) and optical transform function (OTF) of the optical system. The SIM for super-resolution (SIM-SR) proposed by Gustafsson et al. and the SIM for optical sectioning (SIM-OS) proposed by Neil et al. are the most popular ones in the research community of microscopy. They have the same optical configuration, but with different data post-processing algorithms. We mathematically described the basic theories for both of the SIMs, respectively, and presented some numerical simulations to show the effects of super-resolution and optical sectioning. Various approaches to generation of structured illumination patterns were reviewed. As an example, a SIM system based on DMD-modulation and LED-illumination was demonstrated. A lateral resolution of 90 nm was achieved with gold nano-particles. The optical sectioning capability of the microscope was demonstrated with Golgi-stained mouse brain neurons, and the sectioning strength of 930 nm was obtained. |
| 文章类型 | Review |
| 关键词 | Microscopy Structured Illumination Super-resolution Optical Sectioning |
| WOS标题词 | Science & Technology |
| DOI | 10.1007/s11434-014-0181-1 |
| 收录类别 | SCI |
| 关键词[WOS] | DYNAMIC SPECKLE ILLUMINATION ; FIELD FLUORESCENCE MICROSCOPY ; STIMULATED-EMISSION-DEPLETION ; STOCHASTIC TRANSFER-FUNCTION ; SPATIAL LIGHT-MODULATOR ; PHASE-SHIFT ESTIMATION ; WIDE-FIELD ; LIVING CELLS ; FULL-FIELD ; RECONSTRUCTION MICROSCOPY |
| 语种 | 英语 |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| WOS记录号 | WOS:000333126800010 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.opt.ac.cn/handle/181661/22179 |
| 专题 | 瞬态光学研究室 |
| 作者单位 | Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian 710119, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dan, Dan,Yao, Baoli,Lei, Ming. Structured illumination microscopy for super-resolution and optical sectioning[J]. CHINESE SCIENCE BULLETIN,2014,59(12):1291-1307. |
| APA | Dan, Dan,Yao, Baoli,&Lei, Ming.(2014).Structured illumination microscopy for super-resolution and optical sectioning.CHINESE SCIENCE BULLETIN,59(12),1291-1307. |
| MLA | Dan, Dan,et al."Structured illumination microscopy for super-resolution and optical sectioning".CHINESE SCIENCE BULLETIN 59.12(2014):1291-1307. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Structured illuminat（1174KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY | 请求全文 | |
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